[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2019
Citations93235681
h-index3826
i10-index243146

 

..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 27, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2022) ::
SJKU 2022, 27(4): 13-26 Back to browse issues page
Antimicrobial Susceptibility Pattern and Mutations of Outer Membrane Porin in Clinical Isolates of Klebsiella pneumoniae
Maryam Bayramnezhad1 , Maryam Ghane 2, Elham Asadi3
1- M.Sc, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
2- Assistant professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran , ghane@iiau.ac.ir
3- Assistant professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
Abstract:   (1441 Views)
Background and Aim: Klebsiella pneumoniae is one of the most common causes of community-acquired and nosocomial infections and has acquired numerous resistance mechanisms over time. The aim of this study was to evaluate the antibiotic resistance pattern of clinical isolates of K. pneumoniae and investigate the mutations in Ompk35 porin as one of the most important mechanisms of resistance to beta-lactam antibiotics.
Materials and Methods: In this cross-sectional correlation study, 100 urinary isolates of K. pneumoniae from the patients admitted to Milad Hospital were evaluated. Antibiotic susceptibility testing was performed using disk diffusion method. Using PCR method, ompK35 genes were detected, sequenced and the mutations were identified by use of Mega software (version 3.7.3). SPSS software version 20 and Chi-square test were used for data analysis.
Results: The highest resistance rate belonged to amikacin (74%) followed by levofloxacin (69%). Resistance rates to cefepime, gentamicin, ciprofloxacin, meropenem, and ceftriaxone were 62, 58, 53, 41, and 35%, respectively. The highest sensitivity rate belonged to imipenem (80%). Numerous mutations were observed in the Ompk35 porin encoding genes. Among the isolates, 70% harbored mutations in Ompk35 protein and two isolates had five mutations simultaneously. There was no significant relationship between resistance to the antibiotics and gender in our study (P>0.05).
Conclusion: Our findings indicated a high prevalence rate of antibiotic resistance and high frequency of porin mutations among K. pneumoniae isolates. Also, the presence of similar mutations in the isolates suggests that they may have a common origin.
Keywords: Klebsiella pneumoniae, Antimicrobial agents, Porin, Drug resistance
Full-Text [PDF 505 kb]   (478 Downloads)    
Type of Study: Original Research | Subject: Microbiology
Received: 2021/08/19 | Accepted: 2021/12/15 | Published: 2022/12/14
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.IAU.PS.REC.1399.195



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bayramnezhad M, Ghane M, Asadi E. Antimicrobial Susceptibility Pattern and Mutations of Outer Membrane Porin in Clinical Isolates of Klebsiella pneumoniae. SJKU 2022; 27 (4) :13-26
URL: http://sjku.muk.ac.ir/article-1-6912-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 27, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2022) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 44 queries by YEKTAWEB 4652