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:: Scientific Journal of Kurdistan University of Medical Sciences- NO 4, 2022 ::
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Antimicrobial Susceptibility Pattern and Mutations of Outer Membrane Porin in Clinical Isolates of Klebsiella pneumoniae
Maryam Bayramnezhad1 , Maryam Ghane 2, Elham Asadi1
1- Islamic Azad University, Islamshahr Branch
2- Islamic Azad University, Islamshahr Branch , ghane@iiau.ac.ir
Abstract:   (152 Views)
Background and Aim: Klebsiella pneumoniae is one of the most common causes of community-acquired and nosocomial infections and has acquired numerous resistance mechanisms over time. The aim of this study was to evaluate the antibiotic resistance pattern of clinical isolates of K. pneumoniae and to investigate mutations in Ompk35 porin as one of the most important mechanisms of resistance to beta-lactam antibiotics.
Materials and Methods: In this cross-sectional correlation study, 100 urinary isolates of K. pneumoniae from patients admitted to Milad Hospital were evaluated. Antibiotic susceptibility testing was performed using disk diffusion method. The ompK35 gene was detected by PCR method, sequenced and mutations were identified using Mega software (version 3.7.3).   For data analysis, SPSS software version 20 and Chi-square test were used.
Results: The highest resistance rate was observed to amikacin (74%) followed by levofloxacin (69%). Resistance to cefepime, gentamicin, ciprofloxacin, meropenem, and ceftriaxone was 62, 58, 53, 41, and 35%, respectively. The highest sensitivity was obtained for imipenem (80%). Numerous mutations were observed in the Ompk35 porin encoding genes. Among the isolates, 70% harbored mutations in Ompk35 protein and two isolates had five mutations simultaneously. There was no significant relationship between resistance to the studied antibiotics and gender.
Conclusion: Our findings indicate a high prevalence of antibiotic resistance and high frequency of porin mutations among K. pneumoniae isolates. Also, the presence of similar mutations between isolates suggests that they may have originated from the same source.
Keywords: Klebsiella pneumoniae, Antimicrobial agents, porin, Drug resistance
     
Type of Study: Original Research | Subject: Microbiology
Received: 2021/08/19 | Accepted: 2021/12/15
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Ethics code: IR.IAU.PS.REC.1399.195


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مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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