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Evaluation of Candida Spp. colonization in respiratory tract of patients hospitalized in surgical and ICU of hospitals in Fasa
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Mohammad Hosein Afsarian1    , Mohammad Reza Ataollahi2 , Yousef Gholampour2 , Zahra Sharafi2 |
1- University of Medical Sciences , afsariyan@gmail.com
2- University of Medical Sciences |
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Abstract: (317 Views) |
Background and Aim: Infections, particularly lung infections, pose significant challenges for patients in the intensive care unit (ICU) and play a crucial role in patient morbidity and mortality. Candida, Aspergillus, and Cryptococcus are key contributors to fungal infections in these patients. This study aimed to determine the prevalence of fungal colonization, specifically Candida species, in hospitalized ICU patients.
Materials and Methods: Fifty bronchoalveolar lavage (BAL) samples from both right and left lungs were collected from twenty-five patients admitted to hospitals in Fasa. Candida strains were isolated and identified in 23 samples (46%) using PCR-RFLP and sequencing. The in vitro susceptibility of these 23 Candida species to four triazole antifungal drugs was tested using the broth microdilution method.
Results: Candida albicans was the most frequently isolated species (n = 9, 39.1%), followed by C. krusei (n = 4, 17.4%), C. tropicalis (n = 3, 13.1%), C. glabrata and C. dubliniensis (n = 2 each, 8.7%), and C. orthopsilosis, C. guilliermondii, and C. famata (n = 1 each, 4.3%). All Candida species were susceptible to the four triazoles, except for four C. krusei strains, which were resistant to fluconazole and showed susceptibility dose-dependent (SDD) response to itraconazole, and two C. glabrata strains, which were SDD to both fluconazole and itraconazole.
Conclusion: Given the rising number of patients at risk for fungal infections and the potential for resistant fungal colonization in ICU settings, it is crucial to prioritize the investigation of these infections and the implementation of advanced diagnostic methods.
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| Keywords: Candida colonization, ICU, PCR-RFLP, Antifungal susceptibility testing |
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Type of Study: Original Research |
Subject:
Parasitology and Mycology
Received: 2024/05/25 | Accepted: 2024/11/26
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