[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2019
Citations93255708
h-index3827
i10-index245148

 

..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 29, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2024) ::
SJKU 2024, 29(1): 9-21 Back to browse issues page
Comparison of the apoptotic and cytotoxic effects of Astragalus hamosus in the two- and three-dimensional cultures of human umbilical vein endothelial cells
Mozaffar Mahmoodi 1, Golara Nasiri2
1- Department of Molecular Medicine and Medical Biotechnology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran , mzffrmahmoodi@gmail.com
2- Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (403 Views)
Background and aim: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides oxygen and nutrients for the tumors. Many drugs have been identified for anti-angiogenic therapy, which is one of the most important drug mechanisms in cancer treatment. Considering similar anti-tumor activity by a similar mechanism in a herbal medicine, Astragalus hamosus (A. hamosus) we compared apoptotic and cytotoxic effects of this herbal medicine on human umbilical vein endothelial cells (HUVECs) between a 3D fibrin gel model  and a 2D culture model.
Materials and Methods: A. hamosus extract was tested for its   cytotoxicity on HUVECs using MTT assay. Flow cytometry was used to examine apoptosis, cell cycle, and proliferation. Also, by qPCR, we assessed the expression of genes related to apoptosis, such as caspase-9, -8, -3, and Bcl-2.
Results: Angiogenic activities of HUVECs were significantly decreased after treatment with IC50 concentration of A. hamosus extract. Flow cytometry analysis revealed that cell cycle arrest in G0/G1 phase in HUVECs in the 3D model higher than in 2D culture. Anti-proliferation activity of the extract decreased the expression of Ki-67, especially in the 3D culture. Also, after A. hamosus treatment, apoptosis level increased in the 3D culture model compared to that in the 2D culture which was confirmed by flow cytometry and qPCR.
Conclusion: Based on our results, A. hamosus extract can be used to treat tumors by inhibiting angiogenesis. Also, 3D fibrin gel can simulate anti-proliferative and anti-apoptotic properties of tumors better than 2D culture.


 
Keywords: Astragalus hamosus, Angiogenesis, Apoptosis.
Full-Text [PDF 513 kb]   (74 Downloads)    
Type of Study: Original Research | Subject: General
Received: 2023/09/22 | Accepted: 2023/11/12 | Published: 2024/04/16
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

mahmoodi M, nasiri G. Comparison of the apoptotic and cytotoxic effects of Astragalus hamosus in the two- and three-dimensional cultures of human umbilical vein endothelial cells. SJKU 2024; 29 (1) :9-21
URL: http://sjku.muk.ac.ir/article-1-8115-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2024) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4657