[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2020
Citations98385290
h-index3825
i10-index254128

 

..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 29, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2024) ::
SJKU 2024, 29(1): 9-21 Back to browse issues page
Comparison of the apoptotic and cytotoxic effects of Astragalus hamosus in the two- and three-dimensional cultures of human umbilical vein endothelial cells
Mozaffar Mahmoodi1 , Golara Nasiri2
1- Department of Molecular Medicine and Medical Biotechnology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran , mzffrmahmoodi@gmail.com
2- Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (888 Views)
Background and aim: Angiogenesis is necessary for solid tumors to grow and metastasize because it provides oxygen and nutrients for the tumors. Many drugs have been identified for anti-angiogenic therapy, which is one of the most important drug mechanisms in cancer treatment. Considering similar anti-tumor activity by a similar mechanism in a herbal medicine, Astragalus hamosus (A. hamosus) we compared apoptotic and cytotoxic effects of this herbal medicine on human umbilical vein endothelial cells (HUVECs) between a 3D fibrin gel model  and a 2D culture model.
Materials and Methods: A. hamosus extract was tested for its   cytotoxicity on HUVECs using MTT assay. Flow cytometry was used to examine apoptosis, cell cycle, and proliferation. Also, by qPCR, we assessed the expression of genes related to apoptosis, such as caspase-9, -8, -3, and Bcl-2.
Results: Angiogenic activities of HUVECs were significantly decreased after treatment with IC50 concentration of A. hamosus extract. Flow cytometry analysis revealed that cell cycle arrest in G0/G1 phase in HUVECs in the 3D model higher than in 2D culture. Anti-proliferation activity of the extract decreased the expression of Ki-67, especially in the 3D culture. Also, after A. hamosus treatment, apoptosis level increased in the 3D culture model compared to that in the 2D culture which was confirmed by flow cytometry and qPCR.
Conclusion: Based on our results, A. hamosus extract can be used to treat tumors by inhibiting angiogenesis. Also, 3D fibrin gel can simulate anti-proliferative and anti-apoptotic properties of tumors better than 2D culture.


 
Keywords: Astragalus hamosus, Angiogenesis, Apoptosis.
Full-Text [PDF 513 kb]   (262 Downloads)    
Type of Study: Original Research | Subject: General
Received: 2023/09/22 | Accepted: 2023/11/12 | Published: 2024/04/16
References
1. Abbastabar H, Hamidifard P, Roustazadeh A, Mousavi SH, Mohseni S, Sepandi M, et al. Relationships between breast cancer and common non-communicable disease risk factors: An ecological study. Asian Pacific Journal of Cancer Prevention. 2013;14(9):5123-5. [DOI:10.7314/APJCP.2013.14.9.5123]
2. Ginsburg O, Bray F, Coleman MP, Vanderpuye V, Eniu A, Kotha SR, et al. The global burden of women's cancers: a grand challenge in global health. The Lancet. 2017;389(10071):847-60. [DOI:10.1016/S0140-6736(16)31392-7] [PMID]
3. Montazeri A, Vahdaninia M, Harirchi I, Harirchi AM, Sajadian A, Khaleghi F, et al. Breast cancer in Iran: need for greater women awareness of warning signs and effective screening methods. Asia Pacific family medicine. 2008;7(1):1-7. [DOI:10.1186/1447-056X-7-6] [PMID]
4. Pollard JW. Trophic macrophages in development and disease. Nature reviews immunology. 2009;9(4):259-70. [DOI:10.1038/nri2528] []
5. Menbari MN, Rahimi K, Ahmadi A, Mohammadi‐Yeganeh S, Elyasi A, Darvishi N, et al. miR‐483‐3p suppresses the proliferation and progression of human triple negative breast cancer cells by targeting the HDAC8> oncogene. Journal of cellular physiology. 2020;235(3):2631-42. [DOI:10.1002/jcp.29167] [PMID]
6. Quail DF, Joyce JA. Microenvironmental regulation of tumor progression and metastasis. Nature medicine. 2013;19(11):1423-37. [DOI:10.1038/nm.3394] [PMID] []
7. Mahmoodi M, Ferdowsi S, Ebrahimi-Barough S, Kamian S, Ai J. Tissue engineering applications in breast cancer. Journal of Medical Engineering & Technology. 2020;44(4):162-8. [DOI:10.1080/03091902.2020.1757771] [PMID]
8. Wei C, Yang C, Wang S, Shi D, Zhang C, Lin X, et al. Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis. Molecular cancer. 2019;18(1):1-23. [DOI:10.1186/s12943-019-0976-4] []
9. Pleşca‐Manea L, Pârvu AE, Pârvu M, Taaˇmaş M, Buia R, Puia M. et al. Effects of Melilotus officinalis on acute inflammation. Phytotherapy research. 2002;16(4):316-9. [DOI:10.1002/ptr.875] [PMID]
10. Braga PC, Dal Sasso M, Culici M, Spallino A, Falchi M, Bertelli A, et al. Antioxidant activity of Calendula officinalis extract: inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopy. Pharmacology. 2009;83(6):348-55. [DOI:10.1159/000217583] [PMID]
11. Narimani R, Tarakemeh A, Moghaddam M, Mahmoodi Sourestani M. Phytochemical variation within aerial parts of ferula cupularis populations, an endangered medicinal plant from Iran. Chemistry & Biodiversity. 2021;18(12):e2100551. [DOI:10.1002/cbdv.202100551] [PMID]
12. Bocci G, Di Paolo A, Danesi R. The pharmacological bases of the antiangiogenic activity of paclitaxel. Angiogenesis. 2013;16:481-92. [DOI:10.1007/s10456-013-9334-0] [PMID] []
13. Greenwell M, Rahman P. Medicinal plants: their use in anticancer treatment. International journal of pharmaceutical sciences and research. 2015;6(10):4103.
14. Tian Q-E, Yan M, Cai H-L, Tan Q-Y, Zhang W-Y. Astragalus polysaccharides can regulate cytokine and P-glycoprotein expression in H22 tumor-bearing mice. World Journal of Gastroenterology: WJG. 2012;18(47):7079. [DOI:10.3748/wjg.v18.i47.7079] []
15. Ansari G, Delbari A, Karimi M, Akbari Kamrani AA, Abolfathi Momtaz Y, Sahaf R. et al. The effect of Melilotous Officinalis oil on the physical function of older adults with mild to moderate knee osteoarthritis: A double-blind randomized controlled trial. Iranian Journal of Ageing. 2019;14(2):132-43.
16. Qi H, Wei L, Han Y, Zhang Q, Lau AS-Y, Rong J. et al. Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2. International journal of oncology. 2010;36(3):725-35. [DOI:10.3892/ijo_00000548] [PMID]
17. Huang C, Xu D, Xia Q, Wang P, Rong C, Su Y. et al. Reversal of P-glycoprotein-mediated multidrug resistance of human hepatic cancer cells by Astragaloside II. Journal of Pharmacy and Pharmacology. 2012;64(12):1741-50. [DOI:10.1111/j.2042-7158.2012.01549.x] [PMID]
18. Zhang D, Zhuang Y, Pan J, Wang H, Li H, Yu Y, et al. Investigation of effects and mechanisms of total flavonoids of Astragalus and calycosin on human erythroleukemia cells. Oxidative medicine and cellular longevity. 2012;2012:1-5. https://doi.org/10.1155/2012/781516 [DOI:10.1155/2012/209843] [PMID] []
19. Umer KH, Zeenat F, Ahmad W, Vakil A. Therapeutics, phytochemistry and pharmacology of Iklilul Malik (Astragalus hamosus Linn): A natural Unani Remedy. International Journal of Herbal Medicine .2017; 5(5): 01-05
20. Krasteva I, Momekov G, Zdraveva P, Konstantinov S, Nikolov S. Antiproliferative effects of a flavonoid and saponins from Astragalus hamosus against human tumor cell lines. Pharmacognosy Magazine. 2008;4(16):269-72.
21. Saleem S, Shaharyar MA, Khusroo MJ, Ahmad P, Rahman RU, Ahmad K, et al. Anticancer potential of rhamnocitrin 4′-β-d-galactopyranoside against N-diethylnitrosamine-induced hepatocellular carcinoma in rats. Molecular and cellular biochemistry. 2013;384:147-53. [DOI:10.1007/s11010-013-1792-6] [PMID]
22. McCulloch M, See C, Shu X-j, Broffman M, Kramer A, Fan W-y, et al. Astragalus-based Chinese herbs and platinum-based chemotherapy for advanced non-small-cell lung cancer: meta-analysis of randomized trials. Journal of Clinical Oncology. 2006;24(3):419-30. [DOI:10.1200/JCO.2005.03.6392]
23. Song M, Yin Y, Zhang J, Zhang B, Bian Z, Quan C, et al. MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1. Protein & cell. 2014;5(11):851-61. [DOI:10.1007/s13238-014-0093-5] [PMID] []
24. Chen W, Yuan Y, Li C, Mao H, Liu B, Jiang X. et al. Modulating tumor extracellular matrix by simultaneous inhibition of two cancer cell receptors. Advanced Materials. 2022;34(10):2109376. [DOI:10.1002/adma.202109376] [PMID]
25. MOSESSON MW. Fibrinogen and fibrin structure and functions. Journal of thrombosis and haemostasis. 2005;3(8):1894-904. [DOI:10.1111/j.1538-7836.2005.01365.x] [PMID]
26. Mahmoodi M, Ebrahimi-Barough S, Kamian S, Azami M, Mehri M, Abdi M, et al. Fabrication and characterization of a three-dimensional fibrin gel model to evaluate anti-proliferative effects of Astragalus hamosus plant extract on breast cancer cells. Asian Pacific journal of cancer prevention: APJCP. 2022;23(2):731. [DOI:10.31557/APJCP.2022.23.2.731] [PMID] []
27. Csupor‐Löffler B, Hajdú Z, Zupkó I, Réthy B, Falkay G, Forgo P, et al. Antiproliferative effect of flavonoids and sesquiterpenoids from Achillea millefolium sl on cultured human tumour cell lines. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2009;23(5):672-6. [DOI:10.1002/ptr.2697] [PMID]
28. Zuo K, Zhang X, Zou J, Li D, Lv Z. Establishment of a paclitaxel resistant human breast cancer cell strain (MCF-7/Taxol) and intracellular paclitaxel binding protein analysis. Journal of International Medical Research. 2010;38(4):1428-35. [DOI:10.1177/147323001003800424]
29. Duval K, Grover H, Han L-H, Mou Y, Pegoraro AF, Fredberg J, et al. Modeling physiological events in 2D vs. 3D cell culture. Physiology. 2017;32(4):266-77. [DOI:10.1152/physiol.00036.2016]
30. Seo J, Do Yoo J, Kim M, Shim G, Oh Y-K, Park R-W, et al. Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs. Experimental & Molecular Medicine. 2021;53(10):1592-601. [DOI:10.1038/s12276-021-00688-7] [PMID]
31. Kniebs C, Luengen AE, Guenther D, Cornelissen CG, Schmitz-Rode T, Jockenhoevel S, et al. Establishment of a pre-vascularized 3D lung cancer model in fibrin gel-influence of hypoxia and cancer-specific therapeutics. Frontiers in bioengineering and biotechnology. 2021;9:1-11. [DOI:10.3389/fbioe.2021.761846] [PMID] []
32. Campisi M, Shin Y, Osaki T, Hajal C, Chiono V, Kamm RD. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Biomaterials. 2018;180:117-29. [DOI:10.1016/j.biomaterials.2018.07.014] [PMID] []
33. Pourmorad F, Hosseinimehr S, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology. 2006;5(11): 1142-1145.
34. Shojaii A, Motaghinejad M, Norouzi S, Motevalian M. Evaluation of anti-inflammatory and analgesic activity of the extract and fractions of Astragalus hamosus in animal models. Iranian journal of pharmaceutical research: IJPR. 2015;14(1):263.
35. Yance Jr DR, Sagar SM. Targeting angiogenesis with integrative cancer therapies. Integrative cancer therapies. 2006;5(1):9-29. [DOI:10.1177/1534735405285562] [PMID]
36. Hoseinkhani Z, Norooznezhad F, Rastegari-Pouyani M, Mansouri K. Medicinal plants extracts with antiangiogenic activity: where is the link? Advanced Pharmaceutical Bulletin. 2020;10(3):370. [DOI:10.34172/apb.2020.045] [PMID] []
37. Subbaraj GK, Kumar YS, Kulanthaivel L. Antiangiogenic role of natural flavonoids and their molecular mechanism: an update. The Egyptian Journal of Internal Medicine. 2021;33(1):1-10. [DOI:10.1186/s43162-021-00056-x]
38. Gacche R. Compensatory angiogenesis and tumor refractoriness. Oncogenesis. 2015;4(6):1-8. [DOI:10.1038/oncsis.2015.14] [PMID] []
39. Li X, Qu L, Dong Y, Han L, Liu E, Fang S, et al. A review of recent research progress on the astragalus genus. Molecules. 2014;19(11):18850-80. [DOI:10.3390/molecules191118850] [PMID] []
40. Khater M, Greco F, Osborn HM. Antiangiogenic activity of flavonoids: a systematic review and meta-analysis. Molecules. 2020;25(20):4712. [DOI:10.3390/molecules25204712] [PMID] []
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

mahmoodi M, nasiri G. Comparison of the apoptotic and cytotoxic effects of Astragalus hamosus in the two- and three-dimensional cultures of human umbilical vein endothelial cells. SJKU 2024; 29 (1) :9-21
URL: http://sjku.muk.ac.ir/article-1-8115-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2024) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 45 queries by YEKTAWEB 4687