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Alpha-Pinene Effect on Passive Avoidance Memory and CDK5 Gene Expression in the Rat Model of Huntington's Disease Induced by 3-Nitropropionic Acid
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Paria Hashemi1    , Mohammad Raman Moloudi2 , Helia Rahmani3 , Zakaria Vahabzadeh4 , Esmael Izadpanah5 |
1- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Neurosciences Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Department of Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran , eizadpanah2000@gmail.com |
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Abstract: (1051 Views) |
Background and Aim: Huntington's disease is a chronic hereditary disorder that causes cognitive and movement defects in affected individuals by progressive destruction of neurons in the cerebral cortex, striatum and the hippocampus. Studies have shown that increased activity of cyclin-dependent kinase-5 (CDK5) plays an important role in the pathogenesis and occurrence of memory impairment in Huntington's disease. Recently, alpha-pinene has been reported to improve learning and memory performance in Alzheimer's and Parkinson's models. Therefore, the aim of this study was to investigate the effect of alpha-pinene on passive avoidance memory and CDK5 gene expression in Huntington's animal model induced by 3-nitro-propionic acid (3-NP).
Materials and Methods: In this study, 40 male rats were randomly divided into 5 groups: sham, 3-NP (10 mg/kg),and 3 other groups receiving 3-NP (10 mg/kg) + alpha-pinene at doses of 1, 5 and 10 mg/kg (for 3 weeks,via intraperitoneal injection). Passive avoidance memory was assessed through the shuttle box device. Then, the expression level of CDK5 gene was measured by RT-qPCR method in brain cortex and hippocampus.
Results: 3-NP injection caused memory impairment by decreasing step through latency (STL). Alpha-pinene at all three doses improved passive avoidance memory performance. Also, 3-NP injection caused a significant increase in CDK5 gene expression level in the brain cortex and hippocampus compared to that in the sham group. The groups which received alpha-pinene at doses of 5 and 10 mg/kg in brain cortex and 1 mg/kg in hippocampus showed decreased expression level of this gene compared to the group that received 3-NP.
Conclusion: The results of this study showed that alpha-pinene improves passive avoidance memory performance probably by reducing the CDK5 gene expression level in Huntington's animal model induced by 3-NP. |
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| Keywords: Memory impairment, Shuttle box, Alpha-pinene, Huntington disease, Cyclin dependent kinase-5, 3-nitropropionic acid |
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Full-Text [PDF 643 kb]
(250 Downloads)
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Type of Study: Original Research |
Subject:
Physiology and neurosciences
Received: 2023/06/3 | Accepted: 2023/09/26 | Published: 2024/04/16
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Hashemi P, Moloudi M R, Rahmani H, Vahabzadeh Z, Izadpanah E. Alpha-Pinene Effect on Passive Avoidance Memory and CDK5 Gene Expression in the Rat Model of Huntington's Disease Induced by 3-Nitropropionic Acid. SJKU 2024; 29 (1) :34-45 URL: http://sjku.muk.ac.ir/article-1-7942-en.html
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