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In Vitro Protective Effects of Aqueous and Alcoholic Extracts of Caryophyllus aromaticus on Reducing H2O2-Induced DNA Damage in Human Mononuclear White Blood Cells
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Mojtaba Kianmehr1    , Seyed Hossein Abtahi Eivary2 , Jafar Hajavi 3 |
1- Department of Medical Physics, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
2- Department of Medical Sciences of Laboratory, Infectious Diseases Research Center, School of Para-Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
3- Department of Microbiology, Faculty of Medicine, Infectious Diseases Research Center, Gonabad University of Medical Science, Gonabad, Iran. , hajavi.jaf@gmail.com |
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Abstract: (762 Views) |
Background and Aim: This study aimed to investigate the protective effects of aqueous and ethanolic extracts of Caryophyllus aromaticus on DNA damage in mononuclear white cells of human blood using the COMET method.
Materials and Methods: Mononuclear white blood cells were isolated from blood samples taken from 15 healthy volunteers. Cells were treated with H2O2 (25, 50, 100, and 200 μM), as well as with aqueous and ethanolic extracts of aerial parts of cloves (0.05, 0.1, 0.5, 1, and 2.5 mg/ml). Finally, to induce DNA damage the cells were incubated in a combination of 100 μM H2O2 with each of the two aqueous and alcoholic extracts at a concentration of 2.5 mg/ml at 4°C for 30 minutes. The extent of DNA migration was measured using the alkaline single cell gel electrophoresis approach assay, and DNA damage was expressed as tail length (µm), percentage of tail DNA, and tail moment (µm).
Results: DNA damage in the mononuclear white blood cells exposed to the combination of hydrogen peroxide with aqueous extract of clove plant was significantly less than that in the mononuclear white blood cells treated with H2O2 alone. Tail length (µm) was 5.30±1.21 versus 21.84±3.91, percentage of tail DNA (%) was 4.29±1.21 versus 16.21±4.18, and tail movement (µm) was 0.24±0.08 versus 2.35±0.72, respectively (p < 0.001).
Conclusion: The results showed that the aqueous extract of clove plant (2.5 mg/ml) can prevent oxidative DNA damage in human mononuclear white blood cells, which is probably due to the presence of antioxidant compounds in the extract.
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| Keywords: COMET assay, DNA damage, Oxidative stress, Caryophyllus aromaticus |
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Full-Text [PDF 648 kb]
(276 Downloads)
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Type of Study: Original Research |
Subject:
Pharmacology
Received: 2022/10/5 | Accepted: 2023/03/6 | Published: 2023/12/6
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1. Sicinska P, Mokra K, Wozniak K, Michałowicz J, Bukowska B. Genotoxic risk assessment and mechanism of DNA damage induced by phthalates and their metabolites in human peripheral blood mononuclear cells. Sci Rep. 2021;11(1):1-13. [ DOI:10.1038/s41598-020-79932-5] [ PMID] [ ] 2. Swift LH, Golsteyn RM. Genotoxic anti-cancer agents and their relationship to DNA damage, mitosis, and checkpoint adaptation in proliferating cancer cells. Int J Mol Sci. 2014;15(3):3403-31. [ DOI:10.3390/ijms15033403] [ PMID] [ ] 3. Barnes JL, Zubair M, John K, Poirier MC, Martin FL. Carcinogens and DNA damage. Biochem Soc Trans. 2018;46(5):1213-24. [ DOI:10.1042/BST20180519] [ PMID] [ ] 4. Moller P. Assessment of reference values for DNA damage detected by the comet assay in human blood cell DNA. Mutat Res Rev Mutat Res. 2006;612(2):84-104. [ DOI:10.1016/j.mrrev.2005.10.001] [ PMID] 5. Faust F, Kassie F, Knasmüller S, Boedecker RH, Mann M, Mersch-Sundermann V. The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res Rev Mutat Res. 2004;566(3):209-29. [ DOI:10.1016/j.mrrev.2003.09.007] [ PMID] 6. Kianmehr M, Amiri M, Ebrahimzadeh-Bideskan A, Hajavi J. DNA damage assessment in the lymphocytes of construction painters by comet assay. Toxicol Ind Health. 2016;32(11):1902-9. [ DOI:10.1177/0748233715597347] [ PMID] 7. Kianmehr M, Hajavi J, Gazeri J. Assessment of DNA damage in blood lymphocytes of bakery workers by comet assay. Toxicol Ind Health. 2017;33(9):726-35. [ DOI:10.1177/0748233717712408] [ PMID] 8. Shahidi F, Liyana-Pathirana CM, Wall DS. Antioxidant activity of white and black sesame seeds and their hull fractions. Food Chemistry. 2006;99(3):478-83. [ DOI:10.1016/j.foodchem.2005.08.009] 9. Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med. 2006;27(1):1-93. [ DOI:10.1016/j.mam.2005.07.008] [ PMID] 10. Bor Z, Arslan R, Bektaş N, Pirildar S, Dönmez AA. Antinociceptive, antiinflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves. Turk J Med Sci. 2012;42(2):315-24. [ DOI:10.3906/sag-1011-1304] 11. Zangiabadi M, Sahari M, Barzegar M, Naghdi Badi H. Zataria multiflora and Bunium persicum essential oils as two natural antioxidants. J Med Plants. 2012;1(41):8-21. 12. Adel M, Sadegh AB, Yeganeh S, Movafagh AN, Saoud IP. Anesthetic efficacy of clove oil, propofol, 2‐phenoxyethanol, and ketamine hydrochloride on Persian Sturgeon, Acipenser persicus, juveniles. J World Aquac Soc. 2016;47(6):812-9. [ DOI:10.1111/jwas.12286] 13. Dwivedi V, Shrivastava R, Hussain S, Ganguly C, Bharadwaj M. Comparative anticancer potential of clove (Syzygium aromaticum)-an Indian spice-against cancer cell lines of various anatomical origin. Asian Pac J Cancer Prev. 2011;12(8):1989-93. 14. Chaieb K, Hajlaoui H, Zmantar T, Kahla‐Nakbi AB, Rouabhia M, Mahdouani K, et al. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytother Res. 2007;21(6):501-6. [ DOI:10.1002/ptr.2124] [ PMID] 15. Kuroda M, Mimaki Y, Ohtomo T, Yamada J, Nishiyama T, Mae T, et al. Hypoglycemic effects of clove (Syzygium aromaticum flower buds) on genetically diabetic KK-A y mice and identification of the active ingredients. J Nat Med. 2012;66(2):394-9. [ DOI:10.1007/s11418-011-0593-z] [ PMID] 16. Shams R, Singh R, Dar A, Pandiselvam R, Rusu A, Trif M. A comprehensive review on clove (Caryophyllus aromaticus L.) essential oil and its significance in the formulation of edible coatings for potential food applications. Front Nutr. 2022;9:987674-. [ DOI:10.3389/fnut.2022.987674] [ PMID] [ ] 17. Abdel‐Wahhab M, Aly S. Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis. J Appl Toxicol. 2005;25(3):218-23. [ DOI:10.1002/jat.1057] [ PMID] 18. Kim I-S, Yang M-R, Lee O-H, Kang S-N. Antioxidant activities of hot water extracts from various spices. Int J Mol Sci. 2011;12(6):4120-31. [ DOI:10.3390/ijms12064120] [ PMID] [ ] 19. Carlsen MH, Halvorsen BL, Holte K, Bohn SK, Dragland S, Sampson L, et al. The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutr J. 2010;9(1):3-14. [ DOI:10.1186/1475-2891-9-3] [ PMID] [ ] 20. Yang J-I, Yeh C-C, Lee J-C, Yi S-C, Huang H-W, Tseng C-N, et al. Aqueous extracts of the edible Gracilaria tenuistipitata are protective against H2O2-induced DNA damage, growth inhibition, and cell cycle arrest. Molecules. 2012;17(6):7241-54. [ DOI:10.3390/molecules17067241] [ PMID] [ ] 21. Boyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol. 1976;5:9-15. [ DOI:10.1111/j.1365-3083.1976.tb03851.x] 22. Dagur PK, McCoy Jr JP. Collection, storage, and preparation of human blood cells. Curr Protoc Cytom. 2015;73(1):5-16. [ DOI:10.1002/0471142956.cy0501s73] [ PMID] [ ] 23. Lowes LE, Allan AL. Circulating tumor cells and implications of the epithelial-to-mesenchymal transition. Adv Clin Chem. 2018;83:121-81. [ DOI:10.1016/bs.acc.2017.10.004] [ PMID] 24. Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res Rev Mutat Res. 2008;659(1-2):93-108. [ DOI:10.1016/j.mrrev.2008.03.007] [ PMID] 25. Aslanturk OS. In vitro cytotoxicity and cell viability assays: principles, advantages, and disadvantages. Genotoxicity-A predictable risk to our actual world. 2018;2:64-80. [ DOI:10.5772/intechopen.71923] 26. Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10(3):178-82. [ DOI:10.38212/2224-6614.2748] 27. Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007;2(4):875-7. [ DOI:10.1038/nprot.2007.102] [ PMID] 28. Singh RK, Mishra SK, Kumar N, Singh AK. Assessment of DNA damage by comet assay in lymphocytes of workers occupationally exposed to petroleum fumes. Int J Genet. 2010;2(1):18. [ DOI:10.9735/0975-2862.2.1.18-22] 29. Thirugnanasampandan R, Jayakumar R. Protection of cadmium chloride induced DNA damage by Lamiaceae plants. Asian Pac J Trop Biomed. 2011;1(5):391-4. [ DOI:10.1016/S2221-1691(11)60086-5] [ PMID] 30. Mata A, Proença C, Ferreira A, Serralheiro M, Nogueira J, Araújo M. Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chem. 2007;103(3):778-86. [ DOI:10.1016/j.foodchem.2006.09.017] 31. Nabavi S, Nabavi S, Ebrahimzadeh M, Eslami B. In Vitro Antioxidant Activity of Pyrus Boissieriana, Diospyros Lotus, Eryngium Caucasicum and Froriepia Subpinnata. JRUMS. 2009;8(2):139-50. 32. Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, et al. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food chem. 2007;103(4):1449-56. [ DOI:10.1016/j.foodchem.2006.10.061] 33. Miranda DD, Arçari DP, Pedrazzoli J, Carvalho PdO, Cerutti SM, Bastos DH, et al. Protective effects of mate tea (Ilex paraguariensis) on H2O2-induced DNA damage and DNA repair in mice. Mutagenesis. 2008;23(4):261-5. [ DOI:10.1093/mutage/gen011] [ PMID] 34. Sheng Y, Li L, Holmgren K, Pero R. DNA repair enhancement of aqueous extracts of Uncaria tomentosa in a human volunteer study. Phytomedicine. 2001;8(4):275-82. [ DOI:10.1078/0944-7113-00045] [ PMID] 35. Li A, Schuermann D, Gallego F, Kovalchuk I, Tinland B. Repair of damaged DNA by Arabidopsis cell extract. Plant Cell. 2002;14(1):263-73. [ DOI:10.1105/tpc.010258] [ PMID] [ ] 36. Serpeloni JM, Bisarro dos Reis M, Rodrigues J, Campaner dos Santos L, Vilegas W, Varanda EA, et al. In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test. Mutagenesis. 2008;23(6):501-7. [ DOI:10.1093/mutage/gen043] [ PMID] 37. Soltani F, Mosaffa F, Iranshahi M, Karimi G, Malekaneh M, Haghighi F, et al. Evaluation of antigenotoxicity effects of umbelliprenin on human peripheral lymphocytes exposed to oxidative stress. Cell Biol Toxicol. 2009;25(3):291-6. [ DOI:10.1007/s10565-008-9083-9] [ PMID] 38. Yen GC, Hsieh CL. Inhibitory effect of Eucommia ulmoides Oliv. on oxidative DNA damage in lymphocytes induced by H2O2. Teratog Carcinog Mutagen. 2003;23(S1):23-34. [ DOI:10.1002/tcm.10047] [ PMID] 39. Behravan J, Mosafa F, Soudmand N, Taghiabadi E, Razavi BM, Karimi G. Protective effects of aqueous and ethanolic extracts of Portulaca oleracea L. aerial parts on H2O2-induced DNA damage in lymphocytes by comet assay. J Acupunct Meridian Stud. 2011;4(3):193-7. [ DOI:10.1016/j.jams.2011.09.008] [ PMID] 40. Bajpayee M, Dhawan A, Parmar D, Pandey AK, Mathur N, Seth PK. Gender-related differences in basal DNA damage in lymphocytes of a healthy Indian population using the alkaline Comet assay. Mutat Res Genet Toxicol Environ Mutagen. 2002;520(1-2):83-91. [ DOI:10.1016/S1383-5718(02)00175-4] [ PMID] |
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Kianmehr M, Abtahi Eivary S H, hajavi J. In Vitro Protective Effects of Aqueous and Alcoholic Extracts of Caryophyllus aromaticus on Reducing H2O2-Induced DNA Damage in Human Mononuclear White Blood Cells. SJKU 2023; 28 (5) :37-50 URL: http://sjku.muk.ac.ir/article-1-7605-en.html
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