[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2021) ::
SJKU 2021, 26(3): 37-51 Back to browse issues page
Comparison of immunochromatography, thin layer chromatography and gas chromatography techniques for drug detection in urine
Mohammad Ahmadia khosroshahi1 , Parvin Gharbani 2
1- Department of Toxicology, Ahar Branch, Islamic Azad University, Ahar, Iran
2- Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran , PARVINGHARBANI@YAHOO.COM
Abstract:   (274 Views)
Background and Aim: Nowadays, drug abuse is a major problem in societies, and detection of drugs in urine is very important.  In general, immunochromatography (ICG) and thin-layer chromatography (TLC) are routine methods for the detection of drugs in urine. These methods are rapid and economical, but their accuracy rates for the detection of drugs at lower concentrations are low (< cut off). Morphine, amphetamine, and methamphetamine are conventional drugs that are widely used. The purpose of this study was to compare immunochromatography, thin layer chromatography (TLC), and gas chromatography (GC) methods for detection of morphine, amphetamine, and methamphetamine in the spiked healthy human urine samples and urine of addicted people. We also compared their detection limits with one another.
Materials and Methods: This was an experimental study and included urine samples obtained from healthy and addicted people referring to the laboratory of the 7th of Tir marriage counseling center in East Azerbaijan Province, in June 2016. After the collection of urine samples, samples obtained from healthy people were spiked with various concentrations of morphine, amphetamine, and methamphetamine. Then, all samples were tested by immunochromatography, TLC, and GC methods for the detection of morphine, amphetamine, and methamphetamine.
Results: Results showed that ICG and TLC methods can not detect lower concentrations (< cut off) of morphine, amphetamine, and methamphetamine. While, GC can easily detect them in urine samples, even in lower concentrations (< cut off), and has a high detection limit and accuracy rate.
Conclusion: It can be concluded GC method is a powerful and accurate technique for the detection of drugs in urine samples. 
Keywords: Amphetamine, GC, ICG, Methamphetamine, Morphine, TLC
Full-Text [PDF 590 kb]   (118 Downloads)    
Type of Study: Applicable | Subject: General
Received: 2018/11/30 | Accepted: 2020/10/3 | Published: 2021/07/23
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadia khosroshahi M, Gharbani P. Comparison of immunochromatography, thin layer chromatography and gas chromatography techniques for drug detection in urine. SJKU. 2021; 26 (3) :37-51
URL: http://sjku.muk.ac.ir/article-1-4568-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2021) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4331