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In vitro investigation of synergistic effects of sophoraflavanon G extracted from Sophpra pachycarpa in combination with cisplatin/docetaxel on oral squamous cell carcinoma
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Manouchehr Teymouri1    , Negar Abdolahzadeh2 , Saied Mahmoodi3 , Fatemeh Didari Meymand3 , Milad Iranshahi4 , Javid Rasekhi3 , Mahla Abdari5 , Alireza Afshari-Safavi6 , Javad Hashemi7 , Fahimeh Khaghani3 , Samaneh Mollazadeh8 |
1- . Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran & Department of Medical Nanotechnology, School of Medicine, North Khorasan University of Sciences, Bojnurd, Iran
2- Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- School of Dentistry, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5- Comprehensive Research Laboratory, North Khorasan University of Medical Sciences, Bojnurd, Iran
6- Department of Biostatistics and Epidemiology, School of Health, North Khorasan University of Medical Sciences, Bojnurd, Iran
7- Department of Clinical Biochemistry, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
8- Department of Medical Biotechnology, School of Medicine, North Khorasan University of Sciences, Bojnurd, Iran & Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran , samanehmollazadeh@yahoo.com |
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Abstract: (945 Views) |
Background and Aim: Oral squamous cell carcinoma is the most common neoplasm in the oral cavity, accounting for 90% of all oral cavity cancers. It has been shown that natural compounds could exert significant anti-cancer effects on malignant cells. Amongst Sophora species, Sophora pachycarpa, a source of flavonoid compounds such as sophoraflavanone G (SG), possesses a wide range of biological properties, including anticancer effects. Therefore, this study was conducted to investigate the cytotoxic effects of SG extracted from S. pachycarpa, in combination with cisplatin or docetaxel, on oral squamous cell carcinoma cells.
Materials and Methods: SG was extracted from S. pachycarpa with more than 95% purity. The MTT assay was used to determine the cytotoxic effects of the drugs. To investigate the combinatorial effects of SG and drugs on KB cells, the combination index (CI) was evaluated using CompuSyn software.
Results: SG exhibited considerable cytotoxic effects on KB cells compared with HEK-293 cells. In normal cells, this compound enhanced the anticancer activity of cisplatin/docetaxel against KB cells. The combination index showed that SG and cisplatin/docetaxel at low combination concentrations produced synergistic cytotoxic effects on KB cells, with values between 0.3 and 0.4. Alongside, SG+cisPt/DTX caused noticeable morphological changes that could be considered an apoptotic indicator.
Conclusion: These results suggest the potential of SG to enhance the anticancer activity of cisplatin/docetaxel against KB cell lines. It also reduces the cytotoxic effects of cisPt/DTX on HEK-29 cells. Therefore, SG could be a promising candidate for the targeted treatment of oral cancer. |
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| Keywords: Cell Cytotoxicity, Cisplatin, Combinational Effects, Docetaxel, KB Cell Line, Sophoraflavanone G, Sophora pachycarpa, Squamous Cell Carcinoma |
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Full-Text [PDF 1736 kb]
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Type of Study: Original Research |
Subject:
Biotechnology
Received: 2024/05/28 | Accepted: 2026/01/13 | Published: 2026/09/8
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Research code: 4000193, 4000192
Teymouri M, Abdolahzadeh N, Mahmoodi S, Didari Meymand F, Iranshahi M, Rasekhi J, et al . In vitro investigation of synergistic effects of sophoraflavanon G extracted from Sophpra pachycarpa in combination with cisplatin/docetaxel on oral squamous cell carcinoma. SJKU 2026; 31 (3) URL: http://sjku.muk.ac.ir/article-1-8440-en.html
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