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The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats
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Maryam Arabloei Sani1    , Zahra Hajebrahimi2 , Parichehreh Yaghmaei1 , Nasim Hayati Roodbari1 |
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran , hajebrahimi@ari.ac.ir |
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Abstract: (821 Views) |
Background and Aim: Diabetes is a main metabolic disorder in middle-aged and older people. Oxidative stress can interfere with insulin signaling and are directly relevant to the incidence of diabetes. The AKT signaling pathways plays a critical role in regulating glucose homeostasis and proliferation of β cells. P-cymene is an aromatic monotropene with antioxidant and anti-inflammatory properties. In the present study, the therapeutic effects of p-cymene on blood glucose and insulin levels, the antioxidant power of serum (FRAP assay) and the expression of AKT mRNAs in streptozotocin-induced diabetic rats were investigated.
Materials and Methods: Diabetes was induced using the injection of 55 mg/kg streptozotocin in male Wistar rats. Histological, biochemical, and real-time PCR analyses were done to study the effects of metformin (55 mg/kg) and p-cymene (25, 50, and 100 mg/kg) on levels of glucose, insulin, oxidative stress status, and the expression of AKT mRNA.
Results: Streptozotocin increased serum levels of glucose and decreased serum levels of insulin, the antioxidant power of serum, pancreas levels of AKT mRNA, and β island size. Administration of metformin or p-cymene improved the levels of glucose, insulin, AKT mRNA, β island size, and antioxidant power in an independent manner of dose.
Conclusion: These results suggest that p-cymene has hypoglycemic, hyperinsulinemia, and antioxidant properties. It can regulate Akt signaling pathway. Therefore, it may be suggested for the diabetes therapy alone or in combination with metformin.
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| Keywords: Diabetes Mellitus, P-Cymene, Oxidative Stress, AKT |
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Full-Text [PDF 676 kb]
(180 Downloads)
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Type of Study: Original Research |
Subject:
Pharmacology
Received: 2024/01/17 | Accepted: 2024/05/26 | Published: 2024/12/29
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Research code: -- Ethics code: IR.IAU.SRB.REC.1396.168
Arabloei Sani M, Hajebrahimi Z, Yaghmaei P, Hayati Roodbari N. The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats. SJKU 2024; 29 (5) :25-36 URL: http://sjku.muk.ac.ir/article-1-8288-en.html
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