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Isolation of Bacteriophage Effective against Shigella sonnei in Hospital and Municipal Wastewaters
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Serveh Molai1  , Mazaher Khodabandehloo 2, Hime Salimizand3 |
1- MSc student of Medical Microbiology, Department of Microbiology, Faculty of Medicine, Student of Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Associate professor of Medical Virology, Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. , mazaher-kh@muk.ac.ir
3- Assistant professor of Medical Bacteriology, Liver & Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran |
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Abstract: (105 Views) |
Background and Aim: Infections of antibiotic-resistant bacteria make their treatment difficult and sometimes impossible. So, scientists are looking for new solutions to fight these bacteria. Phage therapy can replace antibiotic therapy, especially in multidrug-resistant bacteria. Therefore, we aimed to isolate bacteriophage that is effective against common gram-negative bacteria.
Materials and Methods: In this experimental study, 250 ml of hospital and municipal wastewater samples were gathered. The samples were centrifuged at 4000 rpm for 10 minutes, the supernatants were filtered through a 0.22 μm filter. To phage enrichment, 50 ml of filtered water twice the volume of nutrient broth and host bacteria were incubated at 37 C for 24 hours in a shaker incubator. The double-layer agar method was used to see bacteriophage plaque. Transmission electron microscopy was used to investigate the characteristics of the isolated bacteriophage.
Results: Lytic bacteriophage effective to Shigella sonei was found by testing on municipal wastewater. Electron microscopy showed that the bacteriophage belonged to the family Microviridae. However, bacteriophage against other Gram-negative bacteria was not found in the test on hospital wastewater samples.
Conclusion: The found bacteriophage had a specific lytic effect against Shigella sonei, this bacteriophage could be used for the study of phage therapy for Shigella infection with antibiotic resistance.
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| Keywords: Bacteriophage, Antibacterial Effect, Multidrug Resistant Bacteria, Shigella, Phage Therapy |
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Full-Text [PDF 1059 kb]
(20 Downloads)
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Type of Study: Original Research |
Subject:
Microbiology
Received: 2021/05/10 | Accepted: 2021/10/31 | Published: 2023/03/15
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