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Evaluation of the Effect of Topical Pistachio Hull Hydroalcoholic Extract Ointment on Wound Healing in Rat: A Histologic and Planimetric Study
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Masoumeh Pourjabali1    , Leila Zarei2 , Vahid Ezzati Sarai3 |
1- Assistant Professor, Department of Pathology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
2- Associate Professor, Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. , leilazarei652@yahoo.com
3- Medical Student, Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran |
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Abstract: (2500 Views) |
Background and Aim: Pistachio nut and hull have anti-inflammatory, antimicrobial and antioxidant effects. The aim of this study was to investigate the effects of pistachio hull hydroalcoholic extract ointment on wound healing process.
Materials and methods: For wound planimetric assessment, 20 healthy rats were randomly divided into 5 groups of 4, including control group (open wound without any intervention and treatment), positive control group (open wound with basic ointment without extract), and the 3 other groups receiving 1%, 2% and 4% ointments respectively. On the 7th and 14th days, biopsies from wound tissues were taken and after Mason Trichrome staining we evaluated collagen. H&E staining was done for histopathological assessments.
Results: The results showed that wound treatment with pistachio hull ointment in the experimental groups showed a significant increase (P<0.05) in the percentage of wound healing rate. Also, there was a significant difference in the thickness of skin and collagen diameter, and reduction of edema between experimental and control groups (P<0.05). Also we found significant decrease in inflammatory cell count on the 14th day in the groups treated with pistachio hull extract compared to those in the control group (P<0.05), which was indicative of accelerated wound healing in the experimental groups.
Conclusion: According to the results of this study, pistachio hull hydroalcoholic extract ointment potentially possesses healing effects on the wound created in the skin of the rat.
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| Keywords: Ointments, Medicinal plants, Plant extracts, Staining |
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Full-Text [PDF 676 kb]
(835 Downloads)
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Type of Study: Original Research |
Subject:
Pharmacology
Received: 2021/05/3 | Accepted: 2021/11/28 | Published: 2022/09/11
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