1- PhD Comparative histology, Sina Hospital, Kurdistan University of Medical Sciences, Kamyaran, Iran , Mozafari_49@yahoo.com 2- Histology and Science Department, Faculty of Veterinary, Urmia University, Urmia, Iran. 3- Anatomy and Embryology Science Department, Faculty of Veterinary, Urmia University, Urmia, Iran. 4- Pharmacology and Toxicology Ward, Faculty of Veterinary, Urmia University, Urmia, Iran. 5- Epidemiology Department, Faculty of Veterinary, Urmia University, Urmia, Iran.
Abstract: (5068 Views)
Background and Aim: Oxidative stress can lead to change in the sperm parameters and cessation of embryological development This study aimed to assess the protective effect of ethylpyruvate (EP) on sperm parameters andtrend of in vitro fertilization under oxidative stress conditions produced by phenyl hydrazine (PHZ) induced hemolytic anemia in mice.
Material and Method: 40 NMRI mice with the age range of 8-10 weeks and mean weight of 26±2gr, were randomly divided into four equally groups. The control group received normal saline (0. 1 ml/day, IP). Group2 (PHZ group) was treated with initial dose of PHZ (8mg/100gr/b. w, IP) followed by 6mg/100gr/b. w, IP, every 48hr. Group3, (Group PHZ+EP) received the same dose of PHZ and EP (40mg/kg/daily/IP). Ethyl pyruvate group received only EP (40mg/kg/daily, IP). Treatment period took 35 days. Then, after euthanasia the sperm were collected from caudal region of epididymis and examined for sperm count, sperm viability, motility and morphology. Testis tissue MDA and serum testosterone levels of all experimental groups were also evaluated.
Result: In this study, in PHZ group we found a considerable reduction in the mean percentage of the number of the sperms with damaged DNA and abnormal morphology compared to the control group. After administration of antioxidant these parameters improved significantly (p<0.05). InPHZ group we found significant decreasein the percentage of fertility, blastocysts, and the number of arrested embryos in comparison to the control group, which after administration ofethylpyruvate these parameters improved significantly.
Conclusion: Treatment of the mice with PHZ led to improvement of the sperm parameters and trend of embryological development.
Mozaffari A A, Shahrooz R, Ahmadi A, Malekinejad H, Mardani K. Assessment of protective effect of ethyl pyruvate on sperm parameters, and trend of embryological development after in vitro fertilization (IVF) in phenylhydrazine treated mice. SJKU 2017; 22 (1) :11-24 URL: http://sjku.muk.ac.ir/article-1-2956-en.html