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:: Volume 22, Number 1 (Scintific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(1): 11-24 Back to browse issues page
Assessment of protective effect of ethyl pyruvate on sperm parameters, and trend of embryological development after in vitro fertilization (IVF) in phenylhydrazine treated mice
Dr Ali Akbar Mozaffari 1, Dr Rasoul Shahrooz2, Dr Abbas Ahmadi3, Dr Hasan Malekinejad4, Dr Karim Mardani5
1- PhD Comparative histology, Sina Hospital, Kurdistan University of Medical Sciences, Kamyaran, Iran , Mozafari_49@yahoo.com
2- Associate Professor Histology and Science Department, Faculty of Veterinary, Urmia University, Urmia, Iran.
3- Assistant Professor Anatomy and Embryology Science Department, Faculty of Veterinary, Urmia University, Urmia, Iran.
4- Professor Pharmacology and Toxicology Ward, Faculty of Veterinary, Urmia University, Urmia, Iran.
5- Associate Professor Epidemiology Department, Faculty of Veterinary, Urmia University, Urmia, Iran.
Abstract:  

Background and Aim: Oxidative stress can lead to change in the sperm parameters and cessation of embryological development This study aimed to assess the protective effect of ethylpyruvate (EP) on sperm parameters and trend of in vitro fertilization under oxidative stress conditions produced by phenyl hydrazine (PHZ) induced hemolytic anemia in mice.

Material and Method: 40 NMRI mice with the age range of 8-10 weeks and mean weight of 26±2gr, were randomly divided into four equally groups. The control group received normal saline (0. 1 ml/day, IP). Group2 (PHZ group) was treated with initial dose of PHZ (8mg/100gr/b. w, IP) followed by 6mg/100gr/b. w, IP, every 48hr. Group3, (Group PHZ+EP) received the same dose of PHZ and EP (40mg/kg/daily/IP). Ethyl pyruvate group received only EP (40mg/kg/daily, IP). Treatment period took 35 days. Then, after euthanasia the sperm were collected from caudal region of epididymis and examined for sperm count, sperm viability, motility and morphology. Testis tissue MDA and serum testosterone levels of all experimental groups were also evaluated.

Result: In this study, in PHZ group we found a considerable reduction in the mean percentage of the number of the sperms with damaged DNA and abnormal morphology compared to the control group. After administration of antioxidant these parameters improved significantly (p<0.05). In PHZ group we found significant decrease in the percentage of fertility, blastocysts, and the number of arrested embryos in comparison to the control group, which after administration of ethylpyruvate these parameters improved significantly.

Conclusion: Treatment of the mice with PHZ led to improvement of the sperm parameters and trend of embryological development.

Key words: Ethyl pyruvate, Phenylhydrazine, Sperm parameters, Stress oxidative, Mice.

Keywords: Ethyl pyruvate, Phenylhydrazine, Sperm parameters, Stress oxidative, Mice.
Full-Text [PDF 4463 kb]      
Type of Study: Research | Subject: General
Received: 2017/03/14 | Accepted: 2017/03/14 | Published: 2017/03/14
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Volume 22, Number 1 (Scintific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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