[Home ] [Archive]   [ فارسی ]  
:: Volume 21, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016) ::
SJKU 2016, 21(3): 125-136 Back to browse issues page
Comparison of antimicrobial effect of nanostructure poly (amidoamine) dendrimer generation 4 with conventional antibiotics against water resources indigenous bacteria
Afshin Maleki Dr1, Mohammad Ahmadi Jebelli 2, Bagher Hayati 3, Hiua Daraei 4, Neda Darvish 5
1- Associate Professor Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran. (Corresponding Author), Tel: +98-25-37780175 , m.ahmadijebelli@yahoo.com
3- Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
4- Instructor Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
5- Department of Environment and Energy, Tehran Islamic Azad University, Tehran, Iran.
Abstract:  

Background and Aim: Dendrimers have antibacterial properties and can be used against microbial infections as alternative antibacterial agents with minimal side effects.  In this study we evaluated and compared nanostructure macromolecules called Poly (amidoamine) dendrimer generation 4 (PAMAM-G4) with conventional antibiotics in relation to resistance of isolated bacteria from water resources.

Materials and methods: 523 samples were collected from 108 water resources and membrane filter method was used for bacterial species isolation. Resistance of isolated species to amoxicillin, erythromycin, tetracycline and ciprofloxacin antibiotics was determined by measuring zones of inhibition and then compared to the resistance of these species to PAMAM-G4 dendrimer as an antibacterial agent. Also we measured maximum inhibitory concentration (MIC) of dendrimer against isolated bacteria.

Results: 7.4% of samples showed bacterial contamination. Frequency rates of isolated microbial species were as follows: Escherichia coli (28.2%), Proteus (12.8%), Klebsiella (17.9%), Enterobacter (15.3%), Pseudomonas (17.9%), Bacillus subtilis (5.1%) and Staphylococcus aureus (2.56%). Gram-negative species revealed high resistance to PAMAM-G4 dendrimer based on the measurement of zones of inhibition. PAMAM-G4 dendrimer didn't have any effect on inhibition of the growth of Enterobacter and Pseudomonas. MIC's against Escherichia coli, Klebsiella and Proteus were 1250, 500 and 1250 µg/ml respectively. Gram-positive species showed a good response to PAMAM-G4 dendrimer, so that the MIC's for Staphylococcus aureus and Bacillus subtilis were 1 and 2.5 µg/ml, respectively.

Conclusion: Because of increased number of resistant species to conventional antibiotics, PAMAM-G4 can be considered as a potent antibacterial agent against gram-positive bacteria.

Keywords: Poly amidoamine dendrimer, Antibiotic, Antibacterial agent, Drug resistance, Water resources.

Received: Dec 25, 2015      Accepted: Apr 6, 2016

Keywords: Poly amidoamine dendrimer, Antibiotic, Antibacterial agent, Drug resistance, Water resources.
Full-Text [PDF 799 kb]      
Type of Study: Research | Subject: General
Received: 2016/07/31 | Accepted: 2016/07/31 | Published: 2016/07/31
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML   Persian Abstract   Print


Volume 21, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.047 seconds with 789 queries by yektaweb 3531