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:: Volume 20, Number 6 (Scientifis Journal of Kurdistan University of Medical Sciences 2016) ::
SJKU 2016, 20(6): 44-53 Back to browse issues page
Association of HLA-G INDEL polymorphism with systemic lupus erythematosus (SLE) in Guilan Province, in Iran
Leila Asali Salek Moalemi, Fatemeh Asali Salek Moalemi, Dr Zivar Salehi 1, Dr Seyed Habib Zayeni2
1- Professor , geneticzs@yahoo.co.uk
2- Assistant Professor
Abstract:  

Background and Aim: Systemic lupus erythematosus (SLE) is an autoimmune disease that is characterized by the presence of autoantibodies against nuclear antigens. The exact cause of SLE is unknown, but some of these genes are active in the key pathways, including immune complexes, host immune signal transduction, and interferon pathways which have significant roles in the pathogenesis of SLE. Since the classical HLA class I and II molecules are highly involved in the peptide presentation to the components of immune system, the genes that encode these molecules can be primary candidates associated with susceptibility to autoimmune disorders. HLA-G belongs to the family of non-classical HLA class I antigens. The purpose of this study was to evaluate the association of INDEL polymorphism of the 3UTR region of HLA-G gene with SLE.

Material and Methods: In this study, 80 patients with SLE, and 102 healthy individuals were examined. Genomic DNA was extracted from peripheral blood. The genotypes were determined using ARMS-PCR. Data analysis was performed using MedCalc version 12.1.

Results: The frequencies of homozygot genotypes for the presence of (+14bp/+14bp)14bp, hetrozygot genotypes for (-14bp/+14bp), and homozygot genotypes with deletion of (-14bp/-14bp)14bp segments in the healthy individuals were  21.57%, 41.18% and 37.25%, and in the patients were 17.5%, 42.5% and 40% respectively. The most frequent genotype in the healthy individuals and hetrozygot patients was (-14bp/+14bp). We found no statistically significant differences in the genotype distributions between the cases and controls (Ρ>0.05).

Conclusion: In this study INDEL polymorphism of the 3UTR region of HLA-G gene showed no association with systemic lupus erythematosus. However, further studies are required to confirm the validity of these results.

Keywords: Systemic lupus erythematosus, HLA-G gene, INDEL polymorphism, 14bp

 

Received: Jul 12, 2015      Accepted: Nov 23, 2015

Keywords: Systemic lupus erythematosus, HLA-G gene, INDEL polymorphism, 14bp
Full-Text [PDF 574 kb]      
Type of Study: Research | Subject: General
Received: 2016/02/8 | Accepted: 2016/02/8 | Published: 2016/02/8
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Volume 20, Number 6 (Scientifis Journal of Kurdistan University of Medical Sciences 2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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