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The effect of 5-HT2A receptor stimulation on morphine withdrawal symptoms in mice
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Nasrin Hashemi-Firouzi1    , Siamak Shahidi2 , Simin Afshar3 , Parisa Habibi4 , Mahdi Ramezani 5 |
1- Associate Professor, Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran
2- Professor, Department of Physiology and Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran.
3- Assistant Professor, Neurophysiology Research Center, Hamadan University of medical Science, Hamadan, Iran
4- Assistant Professor, Department of Physiology, Hamadan University of medical Science, Iran
5- Assistant Professor, Anatomical Sciences, Hamadan University of medical Science, Hamadan, Iran , m.ramezani@umsha.ac.ir |
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Abstract: (1426 Views) |
Background and Aim: Serotonergic system has a role in morphine dependence and withdrawal symptoms via its receptors. On the other hand, the effect of 5-HT2A receptor on withdrawal symptoms has not been evaluated completely. The aim of this study was to investigate the effect of 5-HT2A receptor stimulation on morphine withdrawal symptoms in male mice.
Materials and Methods: This experimental study included twenty eight male NMRI mice (20-25g). Morphine dependency was induced in a five- day schedule by 6, 16, 26, 36, 46, 56 and 66 mg/kg doses respectively. On the fifth day, 2 hours after a single dose of morphine, naloxone was injected (3 mg/kg) and the scores (from 0 to 3) of withdrawal symptoms including number of jumps, teeth chattering, writing, head shakes, limbs shakes, and percentage of body weight loss were recorded for 30min.The treatment group received different doses of TCB-2 (03, and 2.5mg/kg; Intraperitoneally).
Results: Withdrawal symptoms in the morphine-dependent mice were significantly more frequent than those in the control mice. Different doses of TCB-2 significantly increased some of the morphine withdrawal symptoms in the experimental groups.
Conclusion: The present study indicated that stimulation of 5-HT2A receptor via its agonist exacerbated the morphine withdrawal symptoms. Further studies are recommended to better understand the role of the 5-HT2A receptor in morphine dependence and withdrawal.
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| Keywords: 5-HT2A receptor, Morphine, Withdrawal syndrome, Mice |
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Full-Text [PDF 1229 kb]
(429 Downloads)
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Type of Study: Original Research |
Subject:
Physiology and neurosciences
Received: 2022/04/23 | Accepted: 2022/08/22 | Published: 2023/05/28
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Ethics code: IR.UMSHA.REC.1399.766
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