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:: Scientific Journal of Kurdistan University of Medical Sciences - No 2, 2022 ::
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Evaluation of the effect of nicotinic acid and folic acid on morphology, membrane integrity and quality of sperm chromatin concentration in normozoospermic men during cryopreservation
Raziah Hashemi1 , Farhad Golshan iranpour2 , Gholam reza Dashti 3, Shahla Ishaqi4 , Afsane Jaberi Asl5 , Abol fazl Dashti6
1- Medical doctor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2- Associated Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3- Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , dashti@med.mui.ac.ir
4- Laboratory Technician, Shahid behesti hospital, Isfahan University of Medical Sciences, Isfahan, Iran
5- MSc, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
6- Doctor of Veterinary Medicine, School of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
Abstract:   (294 Views)
Background and Aim: Sperm cryopreservation is an important assisted reproductive technique. However, due to increased production of reactive oxygen species and oxidative stress, it can impair sperm function and reduce quality of fertility. The aim of this study was to evaluate the effect of nicotinic acid and folic acid during cryopreservation on sperm parameters and chromatin concentration for maintaining sperm function in normozoospermic men.
 
Material and Methods: Thirty samples were collected and examined for chromatin quality, viability, membrane integrity, morphology, motility and frozen in 4 groups: control, folic acid (50 nM), nicotinic acid (10 mM) and a combination of both. After cryopreservation, parameters of sperm were evaluated, each group was compared with before freezing and other groups. Chromatin quality was assessed by toluidine blue (TB) staining, viability by eosin-nigrosin staining, membrane integrity by hypo osmotic swelling test, morphology and movement by computer-aided sperm analysis (CASA) software.
 
Results: Chromatin quality, membrane integrity, normal morphology, sperm motility was lower in all groups and immotile sperms were higher in all groups than before cryopreservation (p <0.001). Chromatin quality in Folic acid, Folic acid + Nicotinic acid groups was more and in control was least (p</05). Sperm viability and normal morphology were highest in folic acid group and other groups, lowest in control group (p <0.05). Highest percentage of membrane integrity was in folic acid group and then nicotinic acid + folic acid, nicotinic acid and control groups, respectively (p <0.05). Folic acid played an important role in maintaining sperm parameters.
 
Conclusion: Nicotinic acid and folic acid have a positive effect in maintaining sperm function during cryopreservation
Keywords: Nicotinic acid, Folic acid, Sperm, Chromatin, Cryopreservation.
     
Type of Study: Original Research | Subject: General
Received: 2019/05/22 | Accepted: 2021/06/26
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مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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