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Association of HOTAIR (rs920778 C>T) Polymorphism with Thyroid Cancer Susceptibility in the Northwest of Iran
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Majid Khalili Namarvar1    , Nasser Pouladi2 , Sara Ghaffarin 3 |
1- MSc graduate, Department of Cellular and Molecular Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran
2- Associated Professor, Department of Cellular and Molecular Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
3- Assistant Professor, Department of Cellular and Molecular Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran , s.ghaffarian@azruniv.ac.ir |
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Abstract: (1515 Views) |
ABSTRACT
Background and Aim: HOTAIR is a long noncoding RNA, that is transcribed from the antisense strand of the homeobox C gene and acts as an oncogene. The aim of this study was to assess the association of HOTAIR (rs920778 C>T) gene polymorphism with the risk of thyroid cancer in the northwest of Iran.
Materials and Methods: In this case-control study we investigated association of HOTAIR (rs920778 C>T) gene polymorphism with thyroid cancer susceptibility in a statistical population consisting of 109 patients and 198 healthy controls by PCR-PFLP. Statistical analysis was performed using javastat online statistics package and SPSS V.26.
Results: The frequency rates of TT, CC, and TC genotypes were 46.78%, 29.35%, and 23.85% in the case group, and 38.88%, 20.20%, and 40.90% in the control group respectively. Statistical analysis showed a significant association between TC genotype (p-value=0.003) (OR=0.452, CI 95%=0.268-0.764) and thyroid cancer risk. Also, C and T allele frequency rates, were 41.28% and 58.71% in the case group and 40.65% and 59.34% in the control group respectively. There was no significant relationship between T (p-value=0.088) and C (p-value=0.088) allele frequency rates and thyroid cancer risk in the northwest of Iran.
Conclusion: These findings suggested that rs920778 C>T polymorphism may be associated with increased susceptibility of thyroid cancer in the northwest of Iran. There was no significant association between rs920778C >T polymorphism and patient’s pathological characteristics consisting of age, tumor grade, lymph node involvement, side involved, tumor size, tumor stage and type of thyroid tumor.
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| Keywords: Single nucleotide polymorphism, Thyroid cancer, HOTAIR gene, rs920778C>T, PCR-PFLP |
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Full-Text [PDF 403 kb]
(387 Downloads)
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Type of Study: Original Research |
Subject:
Molecular Medicine and Genetics
Received: 2021/02/23 | Accepted: 2021/12/21 | Published: 2022/12/14
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