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Evaluation Of Protective Effects Of Alginate Hydrogel During
Neurons Transdifferentiated From Bone Marrow Stromal Cells On
Decrease Of Reactive Oxygene Specious
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Leila Salari asl1, Taki Tiraihi  2 |
1- Master of Sciences, Department of Anatomical Sciences, Faculty of Medical sciences, Tarbiat modares university, Tehran, Iran
2- Professor, Department of Anatomical Sciences, Faculty of Medical sciences, Tarbiat modares university, Tehran, Iran , ttiraihi@gmail.com |
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Abstract: (602 Views) |
Background and Aim: Cell therapy is a feasible method for the treatment of spinal cord injury as well as other neurological disorders. Because of the cell death reported to be in the cell transplants, the use of +scaffolds can be useful to improve cell protection, resulting in an increase in their survival and growth, and differentiation. Since alginate hydrogel biopolymer was reported to be effective in treating spinal cord lesions, in this study, we intended to evaluate the protectivity of alginate hydrogel against free radicals in the cultured neural stem cells (NSCs) induced into neuronal phenotype using valproic acid as inducer, and improvement of their survival will be assessed.
Materials and Methods: Bone marrow stromal stem cell-derived neural stem cells encapsulated in alginate hydrogel, were used in this study. The encapsulated cells were induced by valproic acid and the cells were evaluated by immunocytochemical methods and reverse-transcription polymerase chain reaction (RT-PCR). The induction of apoptosis was achieved by hydrogen peroxide (H2O2), and the cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The Protective Effect of alginate hydrogel in front of H2O2 was evaluated by Ferric Reducing Antioxidant Power (FRAP) and MTT. The control groups were two-dimensional (2-D) cultured of the induced NSCs into neuronal phenotype by valproic acid as well as induction of apoptosis by H2O2
Results: The results showed that the viability of neuronal phenotype treated by H2O2 in alginate hydrogel was higher than that of cells cultured under the same condition in 2-D culture, also, the expression of Bcl2 and survivin was higher, while the expression of Bax was lower. Similar results were noticed with the FRAP technique
Conclusion: The results showed that alginate hydrogel has a protective effect on the induced NSCs into neuron-like cells, following induction of apoptosis by H2O2. The results of gene expression and FRAPwere consistent with the above results
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| Keywords: Alginate hydrogel, neuroprotection, 3D culture, induction, neural stem cell, Valproic acid |
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Full-Text [PDF 1242 kb]
(244 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2019/05/6 | Accepted: 2021/10/9 | Published: 2023/03/15
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