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Central Library of Kurdistan University of Medical Sciences
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:: Volume 28, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2023) ::
SJKU 2023, 28(1): 1-19 Back to browse issues page
Evaluation Of Protective Effects Of Alginate Hydrogel During Neurons Transdifferentiated From Bone Marrow Stromal Cells On Decrease Of Reactive Oxygene Specious
Leila Salari asl1 , Taki Tiraihi 2
1- Master of Sciences, Department of Anatomical Sciences, Faculty of Medical sciences, Tarbiat modares university, Tehran, Iran
2- Professor, Department of Anatomical Sciences, Faculty of Medical sciences, Tarbiat modares university, Tehran, Iran , ttiraihi@gmail.com
Abstract:   (1053 Views)
Background and Aim: Cell therapy is a feasible method for the treatment of spinal cord injury as well as other neurological disorders. Because of the cell death reported to be in the cell transplants, the use of +scaffolds can be useful to improve cell protection, resulting in an increase in their survival and growth, and differentiation. Since alginate hydrogel biopolymer was reported to be effective in treating spinal cord lesions, in this study, we intended to evaluate the protectivity of alginate hydrogel against free radicals in the cultured neural stem cells (NSCs) induced into neuronal phenotype using valproic acid as inducer, and improvement of their survival will be assessed.
Materials and Methods: Bone marrow stromal stem cell-derived neural stem cells encapsulated in alginate hydrogel, were used in this study. The encapsulated cells were induced by valproic acid and the cells were evaluated by immunocytochemical methods and reverse-transcription polymerase chain reaction (RT-PCR). The induction of apoptosis was achieved by hydrogen peroxide (H2O2), and the cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The Protective Effect of alginate hydrogel in front of H2O2 was evaluated by Ferric Reducing Antioxidant Power (FRAP) and MTT. The control groups were two-dimensional (2-D) cultured of the induced NSCs into neuronal phenotype by valproic acid as well as induction of apoptosis by H2O2
Results: The results showed that the viability of neuronal phenotype treated by H2O2 in alginate hydrogel was higher than that of cells cultured under the same condition in 2-D culture, also, the expression of Bcl2 and survivin was higher, while the expression of Bax was lower. Similar results were noticed with the FRAP technique
Conclusion: The results showed that alginate hydrogel has a protective effect on the induced NSCs into neuron-like cells, following induction of apoptosis by H2O2. The results of gene expression and FRAPwere consistent with the above results
Keywords: Alginate hydrogel, neuroprotection, 3D culture, induction, neural stem cell, Valproic acid
Full-Text [PDF 1242 kb]   (383 Downloads)    
Type of Study: Original Research | Subject: General
Received: 2019/05/6 | Accepted: 2021/10/9 | Published: 2023/03/15
References
1. Mahoney MJ, Anseth KS. Contrasting effects of collagen and bFGF-2 on neural cell function in degradable synthetic PEG hydrogels. Journal of biomedical materials research Part A. 2007;81(2):269-78. [DOI:10.1002/jbm.a.30970] [PMID]
2. Tatard VM, D'Ippolito G, Diabira S, Valeyev A, Hackman J, McCarthy M, et al. Neurotrophin-directed differentiation of human adult marrow stromal cells to dopaminergic-like neurons. Bone. 2007;40(2):360-73. [DOI:10.1016/j.bone.2006.09.013] [PMID]
3. Xie X, Liu H, Wu J, Chen Y, Yu Z, De Isla N, et al. Rat BMSC infusion was unable to ameliorate inflammatory injuries in tissues of mice with LPS-induced endotoxemia. Bio-medical materials and engineering. 2017;28(s1):S129-S38. [DOI:10.3233/BME-171634] [PMID]
4. Ye Y, Peng YR, Hu SQ, Yan XL, Chen J, Xu T. In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats. Frontiers in neurology. 2016;7:183. [DOI:10.3389/fneur.2016.00183] [PMID] []
5. Wei L, Wei ZZ, Jiang MQ, Mohamad O, Yu SP. Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke. Progress in neurobiology. 2017. [DOI:10.1016/j.pneurobio.2017.03.003] [PMID] []
6. Onifer SM, Rabchevsky AG, Scheff SW. Rat models of traumatic spinal cord injury to assess motor recovery. ILAR journal. 2007;48(4):385-95. [DOI:10.1093/ilar.48.4.385] [PMID]
7. Li X, Yuan Z, Wei X, Li H, Zhao G, Miao J, et al. Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta. Journal of materials science Materials in medicine. 2016;27(4):77. [DOI:10.1007/s10856-016-5684-7] [PMID] []
8. Joosten EA. Biodegradable biomatrices and bridging the injured spinal cord: the corticospinal tract as a proof of principle. Cell and tissue research. 2012;349(1):375-95. [DOI:10.1007/s00441-012-1352-5] [PMID] []
9. Perale G, Rossi F, Sundstrom E, Bacchiega S, Masi M, Forloni G, et al. Hydrogels in spinal cord injury repair strategies. ACS chemical neuroscience. 2011;2(7):336-45. [DOI:10.1021/cn200030w] [PMID] []
10. Matyash M, Despang F, Mandal R, Fiore D, Gelinsky M, Ikonomidou C. Novel soft alginate hydrogel strongly supports neurite growth and protects neurons against oxidative stress. Tissue engineering Part A. 2012;18(1-2):55-66. [DOI:10.1089/ten.tea.2011.0097] [PMID]
11. Abd El-Rehim HA, El-Sawy NM, Hegazy el SA, Soliman el SA, Elbarbary AM. Improvement of antioxidant activity of chitosan by chemical treatment and ionizing radiation. Int J Biol Macromol. 2012;50(2):403-13. [DOI:10.1016/j.ijbiomac.2011.12.021] [PMID]
12. Kelishomi ZH, Goliaei B, Mahdavi H, Nikoofar A, Rahimi M, Moosavi-Movahedi AA, et al. Antioxidant activity of low molecular weight alginate produced by thermal treatment. Food chemistry. 2016;196:897-902. [DOI:10.1016/j.foodchem.2015.09.091] [PMID]
13. Gilgun-Sherki Y, Rosenbaum Z, Melamed E, Offen D. Antioxidant therapy in acute central nervous system injury: current state. Pharmacological reviews. 2002;54(2):271-84. [DOI:10.1124/pr.54.2.271] [PMID]
14. Facchinetti F, Dawson VL, Dawson TM. Free radicals as mediators of neuronal injury. Cellular and molecular neurobiology. 1998;18(6):667-82. https://doi.org/10.1023/A:1020685903186 [DOI:10.1023/A:1020221919154] [PMID]
16. Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A, Nikbin B. Aging of mesenchymal stem cell in vitro. BMC cell biology. 2006;7:14. [DOI:10.1186/1471-2121-7-14] [PMID] []
17. Li XH, Fu YH, Lin QX, Liu ZY, Shan ZX, Deng CY, et al. Induced bone marrow mesenchymal stem cells improve cardiac performance of infarcted rat hearts. Molecular biology reports. 2012;39(2):1333-42. [DOI:10.1007/s11033-011-0867-2] [PMID]
18. Liu HS, Bai XW, Yang Y, Ge LH. [Multilineage potential of pulp stem cells from human young permanent teeth in vitro]. Beijing da xue xue bao Yi xue ban = Journal of Peking University Health sciences. 2007;39(1):41-5.
19. Yang E, Liu N, Tang Y, Hu Y, Zhang P, Pan C, et al. Generation of neurospheres from human adipose-derived stem cells. BioMed research international. 2015;2015:743714. [DOI:10.1155/2015/743714] [PMID] []
20. McLenachan S, Lum MG, Waters MJ, Turnley AM. Growth hormone promotes proliferation of adult neurosphere cultures. Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. 2009;19(3):212-8. [DOI:10.1016/j.ghir.2008.09.003] [PMID]
21. Jensen JB, Parmar M. Strengths and limitations of the neurosphere culture system. Molecular neurobiology. 2006;34(3):153-61. [DOI:10.1385/MN:34:3:153] [PMID]
22. Galli R. The Neurosphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs). Methods in molecular biology (Clifton, NJ). 2019;1953:139-49. [DOI:10.1007/978-1-4939-9145-7_9] [PMID]
23. Rastogi P, Kandasubramanian B. Review of alginate-based hydrogel bioprinting for application in tissue engineering. Biofabrication. 2019;11(4):042001. [DOI:10.1088/1758-5090/ab331e] [PMID]
24. Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor protocols. 2018;2018(6). [DOI:10.1101/pdb.prot095505] [PMID]
25. Chan LL, Smith T, Kumph KA, Kuksin D, Kessel S, Déry O, et al. A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry. Cytotechnology. 2016;68(5):2015-25. [DOI:10.1007/s10616-016-0015-x] [PMID] []
26. Hassanzadeh K, Nikzaban M, Moloudi MR, Izadpanah E. Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors. Iranian journal of basic medical sciences. 2015;18(6):549-54.
27. Abolfathi AA, Vahabzadeh Z, Mahmoodiaghdam N, Vahabzadeh D, Hakhamanesh MS. Effects of taurine and homocysteine on lipid profile and oxidative stress in fructose-fed rats. Scientific Journal of Kurdistan University of Medical Sciences. 2017;22(3):49-59.
28. Khosrobakhsh F, Moloudi MR, Bigdelo M, Rahimi A. Effect of cholestasis on dynamin-related protein 1 (Drp1) gene expression in rat liver. Scientific Journal of Kurdistan University of Medical Sciences. 2017;22(4).
29. Choe G, Kim SW, Park J, Park J, Kim S, Kim YS, et al. Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts. Biomaterials. 2019;225:119513. [DOI:10.1016/j.biomaterials.2019.119513] [PMID]
30. Kerschenmeyer A, Arlov Ø, Malheiro V, Steinwachs M, Rottmar M, Maniura-Weber K, et al. Anti-oxidant and immune-modulatory properties of sulfated alginate derivatives on human chondrocytes and macrophages. Biomaterials science. 2017;5(9):1756-65. [DOI:10.1039/C7BM00341B] [PMID]
31. Khosravizadeh Z, Razavi S, Bahramian H, Kazemi M. The beneficial effect of encapsulated human adipose-derived stem cells in alginate hydrogel on neural differentiation. Journal of biomedical materials research Part B, Applied biomaterials. 2014;102(4):749-55. [DOI:10.1002/jbm.b.33055] [PMID]
32. Ghorbani S, Tiraihi T, Soleimani M. Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction. Journal of biomaterials applications. 2018;32(6):702-15. [DOI:10.1177/0885328217741903] [PMID]
33. Mahoney MJ, Anseth KS. Contrasting effects of collagen and bFGF-2 on neural cell function in degradable synthetic PEG hydrogels. Journal of biomedical materials research Part A. 2007;81(2):269-78. [DOI:10.1002/jbm.a.30970] [PMID]
34. Tatard VM, D'Ippolito G, Diabira S, Valeyev A, Hackman J, McCarthy M, et al. Neurotrophin-directed differentiation of human adult marrow stromal cells to dopaminergic-like neurons. Bone. 2007;40(2):360-73. [DOI:10.1016/j.bone.2006.09.013] [PMID]
35. Xie X, Liu H, Wu J, Chen Y, Yu Z, De Isla N, et al. Rat BMSC infusion was unable to ameliorate inflammatory injuries in tissues of mice with LPS-induced endotoxemia. Bio-medical materials and engineering. 2017;28(s1):S129-S38. [DOI:10.3233/BME-171634] [PMID]
36. Ye Y, Peng YR, Hu SQ, Yan XL, Chen J, Xu T. In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats. Frontiers in neurology. 2016;7:183. [DOI:10.3389/fneur.2016.00183] [PMID] []
37. Wei L, Wei ZZ, Jiang MQ, Mohamad O, Yu SP. Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke. Progress in neurobiology. 2017. [DOI:10.1016/j.pneurobio.2017.03.003] [PMID] []
38. Onifer SM, Rabchevsky AG, Scheff SW. Rat models of traumatic spinal cord injury to assess motor recovery. ILAR journal. 2007;48(4):385-95. [DOI:10.1093/ilar.48.4.385] [PMID]
39. Li X, Yuan Z, Wei X, Li H, Zhao G, Miao J, et al. Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta. Journal of materials science Materials in medicine. 2016;27(4):77. [DOI:10.1007/s10856-016-5684-7] [PMID] []
40. Joosten EA. Biodegradable biomatrices and bridging the injured spinal cord: the corticospinal tract as a proof of principle. Cell and tissue research. 2012;349(1):375-95. [DOI:10.1007/s00441-012-1352-5] [PMID] []
41. Perale G, Rossi F, Sundstrom E, Bacchiega S, Masi M, Forloni G, et al. Hydrogels in spinal cord injury repair strategies. ACS chemical neuroscience. 2011;2(7):336-45. [DOI:10.1021/cn200030w] [PMID] []
42. Matyash M, Despang F, Mandal R, Fiore D, Gelinsky M, Ikonomidou C. Novel soft alginate hydrogel strongly supports neurite growth and protects neurons against oxidative stress. Tissue engineering Part A. 2012;18(1-2):55-66. [DOI:10.1089/ten.tea.2011.0097] [PMID]
43. Abd El-Rehim HA, El-Sawy NM, Hegazy el SA, Soliman el SA, Elbarbary AM. Improvement of antioxidant activity of chitosan by chemical treatment and ionizing radiation. Int J Biol Macromol. 2012;50(2):403-13. [DOI:10.1016/j.ijbiomac.2011.12.021] [PMID]
44. Kelishomi ZH, Goliaei B, Mahdavi H, Nikoofar A, Rahimi M, Moosavi-Movahedi AA, et al. Antioxidant activity of low molecular weight alginate produced by thermal treatment. Food chemistry. 2016;196:897-902. [DOI:10.1016/j.foodchem.2015.09.091] [PMID]
45. Gilgun-Sherki Y, Rosenbaum Z, Melamed E, Offen D. Antioxidant therapy in acute central nervous system injury: current state. Pharmacological reviews. 2002;54(2):271-84. [DOI:10.1124/pr.54.2.271] [PMID]
46. Facchinetti F, Dawson VL, Dawson TM. Free radicals as mediators of neuronal injury. Cellular and molecular neurobiology. 1998;18(6):667-82. https://doi.org/10.1023/A:1020685903186 [DOI:10.1023/A:1020221919154] [PMID]
47. Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A, Nikbin B. Aging of mesenchymal stem cell in vitro. BMC cell biology. 2006;7:14. [DOI:10.1186/1471-2121-7-14] [PMID] []
48. Li XH, Fu YH, Lin QX, Liu ZY, Shan ZX, Deng CY, et al. Induced bone marrow mesenchymal stem cells improve cardiac performance of infarcted rat hearts. Molecular biology reports. 2012;39(2):1333-42. [DOI:10.1007/s11033-011-0867-2] [PMID]
49. Liu HS, Bai XW, Yang Y, Ge LH. [Multilineage potential of pulp stem cells from human young permanent teeth in vitro]. Beijing da xue xue bao Yi xue ban = Journal of Peking University Health sciences. 2007;39(1):41-5.
50. Yang E, Liu N, Tang Y, Hu Y, Zhang P, Pan C, et al. Generation of neurospheres from human adipose-derived stem cells. BioMed research international. 2015;2015:743714. [DOI:10.1155/2015/743714] [PMID] []
51. McLenachan S, Lum MG, Waters MJ, Turnley AM. Growth hormone promotes proliferation of adult neurosphere cultures. Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. 2009;19(3):212-8. [DOI:10.1016/j.ghir.2008.09.003] [PMID]
52. Jensen JB, Parmar M. Strengths and limitations of the neurosphere culture system. Molecular neurobiology. 2006;34(3):153-61. [DOI:10.1385/MN:34:3:153] [PMID]
53. Galli R. The Neurosphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs). Methods in molecular biology (Clifton, NJ). 2019;1953:139-49. [DOI:10.1007/978-1-4939-9145-7_9] [PMID]
54. Rastogi P, Kandasubramanian B. Review of alginate-based hydrogel bioprinting for application in tissue engineering. Biofabrication. 2019;11(4):042001. [DOI:10.1088/1758-5090/ab331e] [PMID]
55. Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor protocols. 2018;2018(6). [DOI:10.1101/pdb.prot095505] [PMID]
56. Chan LL, Smith T, Kumph KA, Kuksin D, Kessel S, Déry O, et al. A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry. Cytotechnology. 2016;68(5):2015-25. [DOI:10.1007/s10616-016-0015-x] [PMID] []
57. Hassanzadeh K, Nikzaban M, Moloudi MR, Izadpanah E. Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors. Iranian journal of basic medical sciences. 2015;18(6):549-54.
58. Abolfathi AA, Vahabzadeh Z, Mahmoodiaghdam N, Vahabzadeh D, Hakhamanesh MS. Effects of taurine and homocysteine on lipid profile and oxidative stress in fructose-fed rats. Scientific Journal of Kurdistan University of Medical Sciences. 2017;22(3):49-59.
59. Khosrobakhsh F, Moloudi MR, Bigdelo M, Rahimi A. Effect of cholestasis on dynamin-related protein 1 (Drp1) gene expression in rat liver. Scientific Journal of Kurdistan University of Medical Sciences. 2017;22(4).
60. Choe G, Kim SW, Park J, Park J, Kim S, Kim YS, et al. Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts. Biomaterials. 2019;225:119513. [DOI:10.1016/j.biomaterials.2019.119513] [PMID]
61. Kerschenmeyer A, Arlov Ø, Malheiro V, Steinwachs M, Rottmar M, Maniura-Weber K, et al. Anti-oxidant and immune-modulatory properties of sulfated alginate derivatives on human chondrocytes and macrophages. Biomaterials science. 2017;5(9):1756-65. [DOI:10.1039/C7BM00341B] [PMID]
62. Khosravizadeh Z, Razavi S, Bahramian H, Kazemi M. The beneficial effect of encapsulated human adipose-derived stem cells in alginate hydrogel on neural differentiation. Journal of biomedical materials research Part B, Applied biomaterials. 2014;102(4):749-55. [DOI:10.1002/jbm.b.33055] [PMID]
63. Ghorbani S, Tiraihi T, Soleimani M. Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction. Journal of biomaterials applications. 2018;32(6):702-15. [DOI:10.1177/0885328217741903] [PMID]
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Salari asl L, Tiraihi T. Evaluation Of Protective Effects Of Alginate Hydrogel During Neurons Transdifferentiated From Bone Marrow Stromal Cells On Decrease Of Reactive Oxygene Specious. SJKU 2023; 28 (1) :1-19
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Volume 28, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2023) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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