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Therpeutic Effects of Human Serum Albumin on Sperm Parameters Content in Non-Obstructive Azoospermia Patients
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Azra Allavese1    , Marya Partovyan2 , Amir Abdolmaleki3 , Mohammad jafar Rezaee4 , Zakaria Vahabzadeh5 , Negin Chavoshenejad6 |
1- Infertility Center of Best Hospital (Faculty Medicine), Kurdistan University of Medical Sciences, Sanandaj, Iran & Infertility Center of Best Hospital (Faculty Medicine), Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. & Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. , mpartovyan@yahoo.com
3- Amir Abdolmaleki. Department of Operating Room, Nahavand School of Allied Medical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. PhD of Anatomical Sciences & Amir Abdolmaleki. Department of Operating Room, Nahavand School of Allied Medical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran. PhD of Anatomical Sciences
4- Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. & Department of Anatomical Sciences, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
5- Associate Professor of Clinical Biochemistry, Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. & Associate Professor of Clinical Biochemistry, Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
6- Department of Anatomical Sciences, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. & Department of Anatomical Sciences, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. |
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Abstract: (736 Views) |
Background and Aim: Infertility is considered as one of the major human health challenges. Approximately, 40% of infertility cases are associated to the azoospermia. Reactive oxidative stress (ROS) damages the spermatozoa and causes male infertility. Human serum albumin (HSA) can potentially reduce DNA damage and increse the sperm capacity for successful fertilization. In the present study we assessed the potential therpeutic effects of HSA on spermatogenic parameters, protamine gene expression and DNA fragmentation index in non-obstructive azoospermic infertile patients.
Materials and Methods: Testicular sperm extraction (TESE) was used to collect the testicular biopsy (to collect sperm samples) from 20 azoospermia patients (20-40 years) on the basis of the inclusion/exclusion criteria. The samples were divided into two groups of control (n=10, no intervention) and treatment (n=10, sperm samples incubated with HSA for 24hrs). Then, various spermatic parameters (motility, morphology, viability), DNA fragmentation, and protamine contents were assessed. The results were evaluated using SPSS software (v.19). p<0.05 was considered significant.
Results: Incubation of sperm with HSA (for 24 hours) can significantly (p<0.05) promote the progressive motility of sperm and lead to increased expression of protamine gene (type I and II). Also, HSA can significantly reduce the rate of sperm DNA fragmentation (p<0.05). Meanwhile, incubation with HSA had no significant effect on sperm morphology and survival rate.
Conclusion: Incubation of sperm extracted from infertile men with non-obstructive azoospermia (for 24 hours) can potentially increase the progressive movement and protamine content of sperm and reduce DNA fragmentation.
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| Keywords: Non-obstructive, Infertile, Azoospermia, Albumin, Protamine, DNA fragmentation |
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Full-Text [PDF 655 kb]
(107 Downloads)
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Type of Study: Original Research |
Subject:
Biology of Reproduction
Received: 2023/12/15 | Accepted: 2024/10/8 | Published: 2025/03/9
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allavese A, partovyan M, abdolmaleki A, rezaee M J, vahabzadeh Z, chavoshenejad N. Therpeutic Effects of Human Serum Albumin on Sperm Parameters Content in Non-Obstructive Azoospermia Patients. SJKU 2025; 29 (6) :36-44 URL: http://sjku.muk.ac.ir/article-1-8243-en.html
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