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Molecular Identification and Determination of Antifungal Susceptibility Profile of Candida Species Isolated from AIDS Patients in Ahwaz City
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Maryam Hatami1    , Maryam Erfaninejad2 , Akbar Hoseinnejad3 , Mahnaz Fatahinia4 |
1- Ahvaz Jundishapur University of Medical Sciences, & MSc in Medical Mycology, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Assistant Professor, Department of Basic Medical Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran & Assistant Professor, Department of Basic Medical Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran
3- Ahvaz Jundishapur University of Medical Sciences, & Ph.D. Candidate in Medical Mycology, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Associate Professor, Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Fatahinia@yahoo.com |
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Abstract: (873 Views) |
Background and Aim: Candidiasis is a common opportunistic infection in HIV/AIDS patients caused by different Candida species. The aim of this study was to do molecular identification and determine the antifungal susceptibility profile of Candida species, isolated from the mouths of AIDS patients by the disk diffusion method.
Materials and Methods: This study included 50 samples collected from the oral cavity of 50 AIDS patients with oropharyngeal candidiasis (OPC) in Ahvaz City. After microscopic and macroscopic identification, these samples were finally identified by PCR RFLP method. For these 50 isolates, antifungal susceptibility testing was performed by the disk diffusion method, based on CLSI-M44-A standard protocol.
Results: In this study, among 50 samples collected from the oral cavity of AIDS patients, we found Candida albicans in 36% (18), Candida glabrata in 30% (15), Candida tropicalis in 26% (13), Candida krusei in 6% (3) and Candida parapsilosis in 2 % (1) of the cases. Our results showed that clotrimazole, amphotericin B, ketoconazole, voriconazole and itraconazole had the highest antifungal effect on Candida species, respectively. While fluconazole showed less antifungal activity compared to the other antifungal drugs.
Conclusion: Considering the better antifungal activity of voriconazole and itraconazole compared to fluconazole and the increasing resistance to fluconazole, which is the main antifungal used in the treatment of AIDS patients with OPC, itraconazole and voriconazole can be used as alternative oral antifungal drugs in the treatment of these patients.
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| Keywords: Candida species, HIV patients, Disk diffusion method, Oropharyngeal candidiasis. |
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Full-Text [PDF 633 kb]
(116 Downloads)
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Type of Study: Original Research |
Subject:
Parasitology and Mycology
Received: 2023/06/17 | Accepted: 2024/04/23 | Published: 2025/03/9
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Research code: 0G-9945 Ethics code: IR.AJUMS.MEDICINE.REC.1399.034
Hatami M, Erfaninejad M, Hoseinnejad A, Fatahinia M. Molecular Identification and Determination of Antifungal Susceptibility Profile of Candida Species Isolated from AIDS Patients in Ahwaz City. SJKU 2025; 29 (6) :13-23 URL: http://sjku.muk.ac.ir/article-1-7955-en.html
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