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Effects of L-serine on oxidative stress indices and histopathological changes of pancreas in streptozotocin-induced diabetic mice
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Mahshad Sheikhi Narani1    , Akram Vatannejad2 , Asma Kheirollahi3 , Farzaneh Ershad Langeroudi3 , Sara Shokrpoor4 , Samira Alizadeh5 , Asie Sadeghi6 |
1- Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2- Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , vatannejad@ut.ac.ir
3- Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4- Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5- Iranian Research Center on Aging, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
6- Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, kerman, Iran |
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Abstract: (1774 Views) |
Background and Aim: Diabetes mellitus is a metabolic disorder with an increasing global prevalence that can lead to premature death. High blood glucose is the main lab. abnormality in diabetes mellitus which occurs as a consequence of disorder in pancreatic insulin secretion or function. L -Serine supplementation regarded as safe by the FDA can improve glucose homeostasis and reduce homeostasis model assessment-estimated insulin resistance (HOMA-IR) and oxidative stress. The aim of this study was to investigate the effects of L-serine intake on oxidative stress indices and histopathologic changes in the pancreas of diabetic mice.
Materials and Methods: In order to conduct this study, 18 c57bl/6 male mice were purchased and divided into 3 groups (control, diabetic control and diabetic mice treated by L-serine(. Diabetes induced by chemical method (streptozotocin, 200 mg/kg). After four weeks of oral administration of L-Serine (approximately 280 mg/day/mouse), animals were euthanized by guillotine and blood samples and pancreas tissues were obtained to determine biochemical parameters, oxidative stress indices and pathological changes.
Results: The results of this study showed that oral administration of the supplement of L-Serine in diabetic mice could help to lower blood sugar levels and could lead to increased catalase enzyme activity (P<0.05) but had no significant effect on the levels of MDA, cholesterol, and triglyceride. On the other hand, histopathological changes showed a slight reduction in diabetes-induced pancreas damage in mice treated with L-serine.
Conclusions: These findings showed that the supplement of L-Serine may have a protective effect against diabetes-induced pancreas damage by lowering blood sugar and improving oxidative stress status.
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| Keywords: Diabetes, L-Serine, Pancreas, Oxidative stress, Histopathology, Amino acid |
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Full-Text [PDF 1273 kb]
(544 Downloads)
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Type of Study: Original Research |
Subject:
Biochemistry and Clinical Laboratory
Received: 2023/01/22 | Accepted: 2023/07/29 | Published: 2023/12/6
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| References |
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Ethics code: IR.UT.VETMED.REC.1401.012
Sheikhi Narani M, Vatannejad A, Kheirollahi A, Ershad Langeroudi F, Shokrpoor S, Alizadeh S et al . Effects of L-serine on oxidative stress indices and histopathological changes of pancreas in streptozotocin-induced diabetic mice. SJKU 2023; 28 (5) :1-12 URL: http://sjku.muk.ac.ir/article-1-7775-en.html
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