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Anti-apoptotic effects of minocycline on doxorubicin-induced cardiotoxicity in male Wistar rats
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Hossein Piri1    , Ehsan Aali2 , Samaneh Keshavarz Hedayati3 , Sanaz Keshavarz Shahbaz4 , Yazdan Naderi5 |
1- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
2- Associate Professor, Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
3- Department of Pharmacology, Faculty of Medicine, Qazvin University of Medical Science, Qazvin, Iran
4- Cellular and Molecular Research Center, Research Institute for Prevention of Noncommunicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran.
5- Cellular and Molecular Research Center, Research Institute for Prevention of Non-communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. , y.naderi@qums.ac.ir |
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Abstract: (1252 Views) |
Background and Aim: Doxorubicin is an effective chemotherapeutic agent. However, use of this drug is limited due to its dose-dependent cardiotoxicity. Activation of apoptotic pathways in myocardial tissue plays an important role in doxorubicin- induced cardiotoxicity. Minocycline is an antibiotic that has anti-apoptotic effects. In this study, we investigated the protective effects of minocycline against doxorubicin-induced cardiac toxicity in male rats.
Materials and Methods: Forty two adult male rats were divided into control (normal saline), doxorubicin (2.5 mg / kg), minocycline (45 and 90 mg / kg) and treatment (doxorubicin + minocycline 45 and 90 mg / kg) groups. Minocycline was injected intraperitoneally, once a day for 3 weeks. Ejection fraction (EF) and fractional shortening (FS) were measured using echocardiography. The activity of caspase 3/7 and the expression of Bax and Bcli were measured by biochemical methods. Bax and Bcl-2 genes expression levels were estimated by real-time PCR.
Results: In the minocycline-treated groups (45 and 90 mg / kg) the activity of caspase 7/3 and the expression of Bax gene were significantly lower and the levels of EF, FS and Bcl-2 expression were significantly higher than those in the doxorubicin group.
Conclusion: Minocycline reduces doxorubicin-induced cardiotoxicity. The anti-apoptotic effects of minocycline may play an important role in its protective effects.
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| Keywords: Doxorubicin, Minocycline, Cardiotoxicity, Apoptosis |
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Full-Text [PDF 891 kb]
(476 Downloads)
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Type of Study: Original Research |
Subject:
Pharmacology
Received: 2021/12/11 | Accepted: 2022/05/10 | Published: 2023/12/6
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Ethics code: IR.QUMS.REC.1397.330
Piri H, Aali E, Keshavarz Hedayati S, Keshavarz Shahbaz S, Naderi Y. Anti-apoptotic effects of minocycline on doxorubicin-induced cardiotoxicity in male Wistar rats. SJKU 2023; 28 (5) :13-24 URL: http://sjku.muk.ac.ir/article-1-7135-en.html
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