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Evaluation of antibacterial properties of alcohol and water extracts of propolis on Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica
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Faegheh Etminani1   , Adibeh Etminani2 , Shoaleh Darvishi3 |
1- Young researchers and Elite Club, Sanandaj branch, Islamic Azad University, Sanandaj, Iran , Faegheh.Etminani@yahoo.com
2- Young researchers and Elite Club, Sanandaj branch, Islamic Azad University, Sanandaj, Iran
3- Department of Food Science &Technology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. |
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Abstract: (4224 Views) |
Background and Aim: Propolis is one of the most important bee products which has antibacterial property. This study was conducted to investigate antibacterial activity of propolis on Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica.
Material and Method: After propolis collection from different parts of Kurdistan Province and preparation of its alcohol and water extracts, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for bacterial strains were determined. Data analysis was carried out by use of SPSS software. To compare mean values we used Duncan test at the significance level of 5%.
Results: Use of alcoholic solvent (96% ethanol and dimethyl sulfoxide) resulted in a greater mean diameter of growth inhibitory zone in comparison to water extract solvent (p<0.05). Inhibitory concentrations (MICs) of alcoholic extract of propolis for Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica were 0.328, 0.656 and 1.31 mg/ml and The MBCs, were 0.328, 0.656 and 1.31 mg/ml respectively. The MICs of dimethyl sulfoxide extract for Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica were 0.656, 1.31 and 1.31 mg/ml and its MBCs for the above mentioned bacteria were 0.656, 1.31 and 1.31 mg/ml respectively. MICs of water extract of propolis for Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica were 1.31, 2.62 and 2.62 and its MBCs for these bacteria were 2.62, 5.25 and 5.25 respectively.
Conclusion: According to the results, alcohol and water extracts of propolis showed significant effects against Bacillus pumilus, Pseudomonas syringae and Serratia plymuthica in laboratory condition.
Keywords: Bacillus,Proplois, Serratia, Pseudomonas.
Received: Nov 21, 2016 Accepted: Jun 14, 2017 |
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| Keywords: Bacillus, Proplois, Serratia, Pseudomonas. |
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Full-Text [PDF 1630 kb]
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Type of Study: Original Research |
Subject:
General
Received: 2017/09/20 | Accepted: 2017/09/20 | Published: 2017/09/20
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