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Evaluation of oxidant effect of aqueous extract of Ruta graveolens on mice ovary
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Aref Hoshiari1   , Gholamreza Najafi2 , Leila Zareai3 |
1- Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , a.hoshyari.vet@gmail.com
2- Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3- Department of Anatomical Science, Faculty of Medicine, Lorestan University of Medical Sciences ,Khorramabad, Iran. |
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Abstract: (5252 Views) |
Background and Aim:Ruta Graveolens (RG) commonly called Sedab, has been known as a medical plant since ancient times. Flavonoids, rutin (quercetin-rutinoside), quercetin, and furanocoumarins are the most common chemicals found in Sedab. Flavonoids have hepatotoxic and cytotoxic effects and the most abundant flavonoid in Sedab is quercetin. Due to its anti-proliferative and pro-apoptosis activities, quercetin can exert negative influences on ovaries and fertility in females. This study was performed to analyze Sedab aqueous extract and evaluate its oxidative effects.
Materials and Methods: This study included 30 female mice. The animals were divided into control and RG groups. Control group received a daily dose of 0.2 mL of normal saline, and Sedab aqueous extract 300mg/kg/day was given to RG group for 14 days. The levels of MDA, NO and TAC were measured and analysis of the extract were performed by HPLC to evaluate its oxidative effects.
Results: Administration of Sedab extract led to a significant increase in the MDA levels in RG group, in the 1st and 2nd weeks and increased levels of TAC in the 2nd and 3rd weeks compared to the results found in the control group. Meanwhile, NO levels increased and TAC levels decreased significantly in Sedab group in the 1st week compared to those in the control group. Analysis revealed that each 10 mg aqueous extract contains 0.017 mg quercetin.
Conclusion: The results of this study suggested that a dose of 300mg/kg Sedab aqueous extract produced oxidative effect on mice ovarian tissue and after discontinuing administration of the extract and subsequent drop of its serum levels, the anti-oxidative effects appeared.
Keywords: Malone di-aldehyde, Nitric oxide, Total anti-oxidative capacity, Anti-fertility, RutaGraveolens, Mouse.
Received: May 23, 2016 Accepted: Jan 24, 2017 |
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| Keywords: Malone di-aldehyde, Nitric oxide, Total anti-oxidative capacity, Anti-fertility, RutaGraveolens, Mouse. |
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Full-Text [PDF 366 kb]
(1573 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2017/07/24 | Accepted: 2017/07/24 | Published: 2017/07/24
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