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Antibiotic resistance pattern and genetic relatedness of Acinetobacter baumannii isolated from respiratory tract infection in western Iran
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Mobina Niknian1    , Kambiz Feyzi1 , Bita Bakhshi2 , Safoura Derakhshan3 |
1- Kurdistan University of Medical Sciences
2- Tarbiat Modares University, Tehran
3- Kurdistan University of Medical Sciences , sderakhshan76@gmail.com |
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Abstract: (137 Views) |
Abstract
Background and Aim: Spread of drug-resistant Acinetobacter baumannii (A. baumannii) causing respiratory tract infections (RTIs) is posing a therapeutic challenge worldwide. The aims of this study were to study the antibiotic resistance in A. baumannii strains isolated from patients with RTIs in western Iran and to determine their genetic relationship.
Materials and Methods: During four months (November 2020 to February 2021), 59 A. baumannii isolates were collected from respiratory samples of patients with RTI hospitalized in two main hospitals in Kermanshah and Sanandaj cities, western Iran. Isolates were characterized for antimicrobial susceptibility by disk diffusion method and presence of main carbapenemase genes (metallo beta-lactamases and OXA-type carbapenemases) by polymerase chain reaction. Molecular typing of the isolates was undertaken by Enterobacterial repetitive intergenic consensus (ERIC) followed by multilocus sequence typing (MLST) (Pasteur scheme).
Results: The carbapenem resistance was high (91.5%) in isolates. The most common carbapenemase was blaVIM gene (89.8%) followed by blaOXA-24-like gene (78%). Almost half of the isolates (49.2%) harbored the blaOXA-23-like and 10.2% of isolates harbored blaOXA-58-like. In 37.3% of isolates the ISAba1 was found upstream of the blaOXA-51-like. The co-existence of OXA-types and metallo beta-lactamase genes was seen in 89.8% of isolates. Most of the isolates from Sanandaj were grouped into one main genotype: Clone D which contained carbapenem-sensitive and -resistant isolates. The largest clone identified in Kermanshah (Clone E) contained the carbapenem-resistant isolates. MLST identified that some isolates of Clone D and Clone E belong to new sequence type (ST) 2237 and ST380, respectively.
Conclusion: In this study a high level of carbapenem resistance and genetic relatedness were found in A. baumannii isolated from RTI. At present, ST380 and ST2237 are not wide-spreading clones, but they might be emerging clones carrying resistance determinants; therefore, awareness about the prevention of spread of these STs in hospitals may be necessary.
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| Keywords: Respiratory tract infections, Acinetobacter baumannii, carbapenem, metallo-beta-lactamase, Bacterial typing |
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Type of Study: Original Research |
Subject:
Microbiology
Received: 2024/10/21 | Accepted: 2026/02/25
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