[Home ] [Archive]   [ فارسی ]  
:: Volume 13, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2008) ::
SJKU 2008, 13(3): 28-34 Back to browse issues page
Evaluation of three enrichment broths and six plating media for isolation and detection of salmonella in food stuffs
Mohammad Mehdi Soltan Dallal Dr * 1, Saeed Vahedi Dr2, Ayoob Ebrahimi , Hamideh Noroz Babaei , Parasto Sadat Fazeli Fard , Fatemeh Saberpoor , Farahnaz Fakhariyan , Akram Sadat Tabatabaei
1- Professor , soltanda@sina.tums.ac.ir
2- Instructor
Abstract:   (10668 Views)

 

Background and Aim: Salmonella is an important enteric pathogen which can transmitted via food and water. Therefore careful and rapid identification of this organism is very important. The aim of this study was to improve the results of the conventional methods for isolation and identification of this microorganism.

Material and Methods: 100 food samples were selected randomly, and incubated in Peptone Water (PW) at 37 degrees C for 24 hours (Pre enrichment). Rappaport -Vassiliadis Broth (RV) at 42°C, Tetrathionate Broth (TT) at 42°C and Fluid Selenite Cystine Broth (SC) at 37°C were the media used for enrichment and isolation of Salmonella. After 24 hours samples  from each method were inoculated into six different media Hektone Enteric agar (HE), Rambach agar (RA), CHROMagar Salmnella agar (CAS), Brilliant Green agar (BG), Salmonella – Shigella agar (SS)and Xylose – Lysine – Deoxycholate agar (XLD) and kept at 37°C for 18-24 hours. The suspected colonies from above mentioned media (3-5 colonies) were selected for biochemical tests in different ional media.

Results:8 samples (8%) were contaminated with Salmonella. 57.4% of colonies in RV, 27.7% in SC and 14.9% in TT were positive. The highest and the lowest rates of isolation were (27.7%) and (6.4%) for RA and BG respectively.

Conclusion: The results of this study showed that among five media used for isolation ofSalmonella, RA medium was the best, while RV medium was suitable for revival of Salmonella.

 

Conflict of Interest: Nill

Received: June 18, 2008      Accepted: August 4, 2008  

Keywords: Salmonella, Foodborne diseases, Isolation.
Full-Text [PDF 132 kb]   (2082 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/04/11
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print



Volume 13, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2008) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 3728