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:: Volume 15, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2010) ::
SJKU 2010, 15(3): 1-12 Back to browse issues page
Decrease in glutamate level in cerebral cortex and lumbar region of spinal cord of rat: a potential mechanism for minocycline in attenuating morphine-induced tolerance
Esmael Izadpanah Dr1, Mahboob Nemati Dr2, Mohammad Charkhpour Dr3, Mohammad Aghazadeh Amiri Dr 4
1- Kurdistan University of Medical Sciences
2- Associate Professor Tabriz University of Medical Sciences
3- Assistan Professor Tabriz University of Medical Sciences
4- Assistan Professor Kurdistan University of Medical Sciences , kambizhassanzadeh@gmail.com
Abstract:   (11560 Views)
ABSTRACT Background and Aim: Chronic opiate administration induces tolerance to the analgesic effect. Despite extensive investigations in this ground, the precise cellular mechanisms underlying opioid tolerance and dependence remain controversial. Several studies have indicated that glutamatergic transmission and nitric oxide/ N-methyl D-aspartate (NMDA) pathway could play an important role in morphine-induced tolerance. The main aim of this study was to evaluate the effects of intra-cerebro-ventricular (ICV) administration of minocycline (a second-generation tetracycline) on morphine-induced tolerance and elevation of glutamate level in cerebral cortex and lumbar region of spinal cord of rats after administration of morphine. Material and Methods: Different groups of rats received either morphine (IP) and distilled water (ICV) or morphine (IP) and different doses of minocycline (ICV) or minocycline alone once per day. Nociception was assessed using a hot plate apparatus. The glutamate concentration in both regions was measured with a high performance liquid chromatography (HPLC) apparatus. Results: The results indicated that ICV administration of minocycline with doses of 60, 120, 240 µg/10µl/rat attenuated the morphine-induced tolerance and decreased glutamate level in the cerebral cortex. But glutamate level in the lumbar spinal cord decreased after administration of minocycline with doses of 120, 240 µg/10µl/rat. Conclusion: We found that central administration of minocycline attenuated morphine-induced increase of glutamate level in the cortex and lumbar spinal cord of rats which can be regarded as a possible mechanism for effect of minocycline on morphine-induced tolerance. Key words: Morphine, Minocycline, Glutamate, Tolerance, Intra-cerebro-ventricular Conflict of Interest: Nill Received: July 6, 2010 Accepted: Sept 27, 2010
Keywords: Morphine, Minocycline, Glutamate, Tolerance, Intra-cerebro-ventricular
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Type of Study: Research | Subject: General
Received: 2011/01/5 | Accepted: 2015/01/10 | Published: 2015/01/10
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Volume 15, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2010) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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