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:: Volume 22, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2018) ::
SJKU 2018, 22(6): 63-73 Back to browse issues page
The ability of H2O2 to induce differentiation of human mesenchymal stem cells into chondrocytes
Farjam Goudarzi 1, Adel Mohammadalipour 2, Majid Bahabadi Dr2, Mohammad Nabi Moradi 1, Arash Sarveazad Dr3, Mohammad Taghi Goodarzi Dr4, Iraj Khodadadi Dr 5
1- Students Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Department of Clinical Biochemistry, Faculty of Medicine, Qazvin University of Medical Sciences, Iran
3- Assistant Professor Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
4- Professor Department of Biochemistry, Hamadan University of Medical Sciences, Hamadan-Iran
5- Associate Professor Department of Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran , ikhodadadi@yahoo.com
Abstract:   (1081 Views)
Background and Aim: Cartilage disorders may deteriorate following oxidative stress injuries affecting mature chondrocytes.  Meantime, mesenchymal stem cells (MSCs) can differentiate into chondrocytes in the presence of oxidative conditions and act as a source of compensation for injured chondrocytes.  The present study aimed to investigate the effect of H2O2 on MSCs differentiation into chondrocytes in order to cast light on the dual roles of oxidative stress in the pathogenesis of diseases.
Materials and Methods: Human mesenchymal stem cells were isolated from abdominal adipose tissue of three different donors and cultured in the presence of 50 mM H2O2 in order to differentiate into chondrocytes.  We determined cell viability by tetrazolium assay and measured reactive oxygen species (ROS) level by flow cytometry. Presence of glycoseaminoglycans was confirmed by safranin staining. 
Results: The percentage of cells containing ROS was significantly higher in the cells treated with hydrogen peroxide (29.2% ± 1) compared to that in the untreated control cells (7.7% ± 1.4).  A significant increase in glycoseaminoglycan content was observed in H2O2 treated cells compared to  that in the  control cells both on the 9th  day (treated: 1.57×104 ± 0.1 vs control: 0.91×104 ± 0.09) and 21st day (treated: 2.87×104 ± 0.2 vs control: 0.96×104 ± 0.07).  In addition, comparison of glycoseaminoglycan content on the 9th and 21st days showed a significantly higher content in both treated and control cells on the 21st  day (p<0.05).
Conclusion: Hydrogen peroxide resulted in increased differentiation of adipose tissue-derived MSCs into chondrocytes.  Therefore, we concluded that, oxidative stress had positive role in the induction of chondrocyte differentiation.
Keywords: Chondrocytes, Differentiation, Glycosaminoglycans, Oxidative stress, Stem cells.
 
Received: Mar 1, 2017     Accepted: Nov 20, 2017
Keywords: Chondrocytes, Differentiation, Glycosaminoglycans, Oxidative stress, Stem cells.
Full-Text [PDF 4661 kb]   (257 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/01/24 | Accepted: 2018/01/24 | Published: 2018/01/24
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Volume 22, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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