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:: Volume 19, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2014) ::
SJKU 2014, 19(4): 79-90 Back to browse issues page
Investigation of the protective effect of epigallocatechingallat on the morphology and viability of the rat bone marrow mesenchymal stem cells following treatment with Bisphenol A
Bayan Lotfi 1, Malek Soleimani Mehranjani Dr2, Majid Mahdiyeh Dr3
1- Arak University , Bayanlotfi@yahoo.com
2- Associate Professor Arak University
3- Assistant Professor Arak University
Abstract:  
Background and Aim: Environmental contaminants such as bisphenol A, in addition to the environmental problems can affect human health. Epigallocatechingallat has been found to have antioxidant and cytoprotective properties in cultured cells but its effect on toxicity induced by bisphenol A has not yet been determined in mesenchymal stem cells. The aim of this investigation was to study the protective role of epigallocatechingallat in rat bone marrow mesenchymal stem cells following treatment with bisphenol A(an oxidative stress inducer). Material and Methods: In this experimental study, rat bone marrow mesenchymal stem cells were extracted using flashing-out method and cultured in DMEM containing 15% FBS and 100U/ml Pen/Strep. At the end of the third passage, cells were divided into 4 groups: control, bisphenol A, bisphenol A + epigallocatechingallat and epigallocatechingallat. The groups were treated for 12, 24, 36, and 48 hrs. After treatment with bisphenol A and epigallocatechingallat, viability, morphology, rate of DNA damage and calcium content of the cells were evaluated. Data were analyzed by one way ANOVA. p<0.05 was considered significant. Results: Bisphenol A caused a significant reduction in the viability and calcium content of the cells. In addition, morphological changes such as nuclear breakage and chromatin condensation, as well as cytoplasm shrinkage, were observed in the group treated with bisphenol A. We found alterations in these parameters in the group of bisphenol A+ epigallocatechingallat which were similar to those observed in the control ones. Conclusion: Epigallocatechingallat can produce a protective role against the toxic effects of bisphenol A in the mesenchymal stem cells. Keywords: Epigallocatechingallat, Bisphenol A, Mesenchymal stem cells. Received: Oct 21, 2013 Accepted: Aug 25, 2014
Keywords: Epigallocatechingallat, Bisphenol A, Mesenchymal stem cells.
Full-Text [PDF 374 kb]      
Type of Study: Research | Subject: General
Received: 2014/12/18 | Accepted: 2014/12/18 | Published: 2014/12/18
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Volume 19, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2014) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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