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:: Volume 14, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2009) ::
SJKU 2009, 14(1): 9-22 Back to browse issues page
Induction of differentiation of bone marrow mesenchymal stem cells of rat into hepatocyte and study of their biochemical factors
Mohammad Abdi 1, Nasrin Sheykh Dr2, Iraj Amiri Dr2, Fardin Fathi Dr3, Masoud Saiidijam Dr2
1- Instructor , m_abdi355@yahoo.com
2- Assistant Professor
3- Associate Professor
Abstract:   (9318 Views)
Background and Aim: Bone marrow mesenchymal stem cells (MSCs) are multi potential and capable of differentiating into specialized tissues. The aim of this study was to investigate the induction of differentiation of the bone marrow MSCs of rat into hepatocytes by use of fibroblast growth factor-2 (bFGF), hepatocyte growth factor (HGF) and oncostatin M (OSM) in order to find a suitable source of hepatoecytes for bioartificial liver or liver transplantation. Material and Methods: In this research MSCs were obtained from the bone marrow of rat and cultured in DMEM-LG medium supplemented with 15% FBS. These cells were treated with differential medium supplemented with HGF, bFGF and OSM for 24 days. Morphology, RT-PCR and Biochemical assays were used to identify the differentiation of stem cells into hepatic cells. Result: MSCs took a round shape after differentiation, while undifferentiated cells had fibroblast-like morphology. Albumin, urea and alpha-fetoprotein (AFP) were detected in differentiated cells. Alkaline phosphatase activity was confirmed by biochemical tests. The mRNA expression of CK-18 and tyrosine amino transferase (TAT) in differentiated cells was demonstrated by RT-PCR after induction. Conclusion: The rat MSCs from bone marrow can differentiate into hepatocyte-like cells in the presence of HGF, bFGF and OSM in vitro. Cytokines may play an important role in differentiation of bone marrow MSCs of rat into hepatocytes. Bone marrow derived MSCs are a new source of cell types which can be used for cell transplantation in hepatic diseases. Conflict of Interest: Nill Received: Feb 8, 2009 Accepted: March 8, 2009
Keywords: Cell differentiation; Hepatocytes; Mesenchymal stem cells (MSCs); Stem cells.
Full-Text [PDF 777 kb]   (1272 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/06/15
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Volume 14, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2009) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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