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Molecular Investigation of Extended-Spectrum Beta-Lactamase Genes of TEM ،PER،SHV، CTX- and GES in clinical isolates of Pseudomonas aeruginosa and Evaluation of Its Relationship with Antimicrobial Resistance
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Razieh Nazari1    , Samaneh Malekshahi2 , Seyed Hasan Adeli3 |
1- Department of Microbiology, Qo.C, Islamic Azad University, Qom, Iran , Razieh.nazari@iau.ac.ir
2- Department of Microbiology, Qo.C, Islamic Azad University, Qom, Iran
3- Department of Internal Medicine, Faculty of Medicine, Medical University of Qom, Qom, I.R. Iran |
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Abstract: (26 Views) |
Background and Aim: Pseudomonas aeruginosa causes numerous therapeutic problems in patients due to the presence of various effective factors in antibiotic resistance such as beta-lactamase enzymes. The aim of this study was to investigate antibiotic resistance pattern in clinical isolates of Pseudomonas aeruginosa and the frequency of beta-lactamase genes of TEM, PER, SHV, CTX-M and GES using PCR.
Materials and Methods: The antibiotic resistance pattern of 150 isolates of Pseudomonas aeruginosa were determined by the Kirby-Bauer method according to CLSI standards. Then, the frequency of beta-lactamase genes of TEM, PER, SHV, CTX-M and GES in the isolates was evaluated using PCR method. The results were analyzed with SPSS software and Pearson's chi-squared test.
Results: The finding of this study showed that, 52% (78 isolates) of Pseudomonas aeruginosa isolates were recovered from tracheal aspirate from ICU wards. Among 150 isolates, the lowest rate of resistance was seen against cefepime (38%) and amikacin (42%). Also, most isolates (78%) showed a minimum inhibitory concentration of ≥512 µg/mL for ceftazidim.The results of genotypic analysis of extended-spectrum beta-lactamases in ceftazidime-resistant isolates showed that the CTX-M gene had the highest frequency and the GES gene had the lowest frequency.
Conclusion: The results of the present study showed that the CTX-M gene seems to play a greater role in antibiotic resistance of Pseudomonas aeruginosa compared to other beta-lactamase genes studied, because the distribution of this gene in resistant isolates was higher than other genes.
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| Keywords: Pseudomonas aeruginosa, Antimicrobial resistance, Beta-lactamase gene, PCR |
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Type of Study: Original Research |
Subject:
Microbiology
Received: 2025/07/13 | Accepted: 2025/12/14
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