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Evaluation of the Efficiency of the POCT Test Used for Measuring Urinary Morphine in the Hospital Laboratories of Sanandaj
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Yashar Mazloumbashiri1    , Mohammad Abdi , Bijan Nouri , Fatemeh Ghalandari |
| 1- , y2ymb@yahoo.com |
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Abstract: (36 Views) |
Background and Objective: The current first-line diagnostic method for detecting drugs of abuse, especially morphine, involves measuring the concentration of this alkaloid using point-of-care (POCT) drug tests in patient urine. The aim of this study was to evaluate the efficacy of this test in comparison with the high-performance liquid chromatography (HPLC) method.
Materials and Methods: This study was conducted on urine samples from individuals suspected of drug abuse or patients undergoing morphine therapy, referred to hospital laboratories in Sanandaj. In this study, a common POCT kit used in laboratories in Sanandaj city, which uses the Lateral Flow Immunoassay (LFIA) method (Hanan Teb Pars Company, Iran), and HPLC method were used to simultaneously measure morphine in samples. Then, to compare the two methods used, the limit of detection (LOD), sensitivity, specificity, and efficiency indices were calculated.
Results: The limit of detection (LOD) for both the HPLC and LFIA methods used in this study was 300 ng/ml. Compared to the HPLC method, the sensitivity and specificity of the LFIA method were 42.65% and 64%, respectively.
Conclusion: The results of this study clearly indicate the low diagnostic value of the LFIA method used in Sanandaj laboratories. Consequently, the inaccurate results obtained from this method can impact patient management and lead to adverse outcomes. The HPLC method established in this study is proposed as an alternative method. |
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| Keywords: Drug abuse, Morphine, Immunochromatography, High Performance Liquid Chromatography(HPLC) |
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Type of Study: Original Research |
Subject:
Biochemistry and Clinical Laboratory
Received: 2024/12/5 | Accepted: 2024/12/30
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