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Protective effects of vitamin E against sertraline-induced oxidative stress on the reproductive system and expression of Bcl-2, Caspase-3 and Hsp70-2 genes in testicular tissue of mice
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Hassan Morovvati    1, Hojat Anbara2 , Fereshteh Morshedi2 |
1- Professor, Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , hmorovvati@ut.ac.ir
2- Ph.D. Candidate, Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran |
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Abstract: (1509 Views) |
Background and Aim: Sertraline is an antidepressant drug and many controversial reports have been presented on the complications of sertraline on the reproductive system. In this study we evaluated the protective effects of vitamin E against sertraline-induced damage to the reproductive system of male mice.
Materials and Methods: In this experimental study, 40 adult male mice were divided into 8 groups of 5. Four groups received 100 IU/kg.bw vitamin E by gavage for 42 days. One group was considered as control and three groups received just sertraline with doses of 5, 10 and 20 mg/kg.bw orally. 24 hours after the last treatment, testes tissue and blood samples were collected and sent for histochemical and biochemical studies, spermatogenic indices as well as measurement of the expressions of Bcl-2, Caspase-3 and Hsp70-2 genes.
Results: The results showed a significant increase in the level of malondialdehyde, nitric oxide and expression of Caspase-3 and Hsp70-2 genes. We also found a significant decrease in spermatogenesis indices, total antioxidant capacity, testosterone and Bcl-2 gene expression in the group receiving 20 mg/kg.bw sertraline (P<0.05). The above mentioned parameters were improved in the groups that received vitamin E along with sertraline.
Conclusion: Vitamin E can decrease the adverse effects of sertraline on biochemical and histochemical parameters, spermatogenic indices and can increase the expression of Bcl-2, Caspase-3 and Hsp70-2 genes in the testicular tissue of mice. |
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| Keywords: Sertraline, Vitamin E, Apoptosis, Bcl-2, Caspase-3, Hsp70-2. |
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Full-Text [PDF 1823 kb]
(740 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2021/07/12 | Accepted: 2021/11/17 | Published: 2023/01/29
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