[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 24, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2019) ::
SJKU 2019, 24(5): 42-55 Back to browse issues page
Microgravity effect on expression of Raf kinase inhibitor protein on MCF-7 breast cancer cell line
Maryam Salavatifar Dr 1, Zahra Hajebrahimi Dr2
1- Aerospace Research Institute, Ministry of Science , salavati@ari.ac.ir, salavatifar2@gmail.com
2- Aerospace Research Institute, Ministry of Science
Abstract:   (797 Views)
Background and Aim: The living organisms on the earth are under the influence of natural gravity force and if this gravitational force changes, they will be affected by a unique shock. Weightlessness has important effects on cell function by interfering with biochemical pathways and gene expressions. Study of these changes would be of benefit to astronauts and can improve the quality of human life on earth. In simulated microgravity, the expression of some genes and protein levels produced in cultured cells or laboratory animals have been altered. However, very little information is available on the effects of microgravity on gene expression. Raf kinase inhibitory protein (RKIP) is a regulator of kinase
activity and a cell balancing agent that can act as a metastatic inhibitor in a variety of solid tumors, including breast cancer. In general, the RKIP expression in progressive tumors is reduced but its increased expression can result in reduced invasive potency of cancer cells without affecting primary tumor growth.
Materials and Methods: In this study, changes of RKIP gene expression were investigated in human MCF-7 breast cancer cells after 24 and 72 hours exposure to microgravity conditions using qReal time PCR method. We used t-test for data analysis and graphpad prism 7 project for plotting graphs.
Results: The results showed that microgravity changed gene expression of RKIP and led to increased expression levels of the gene after 24 hours exposure to microgravity.
Conclusions: By optimizing the duration of microgravity, we can observe significant changes in RKIP gene expression in MCF-7 cell line which can be considered promising for the treatment of cancer.
Keywords: Clinostat, Gravity, RKIP protein, Tumor
Full-Text [PDF 383 kb]   (192 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/02/2 | Accepted: 2019/10/5
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

salavatifar M, hajebrahimi Z. Microgravity effect on expression of Raf kinase inhibitor protein on MCF-7 breast cancer cell line. SJKU. 2019; 24 (5) :42-55
URL: http://sjku.muk.ac.ir/article-1-4769-en.html


Volume 24, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2019) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4106