1- Shahid Beheshti Universityof Medical Sciences 2- Shahid Beheshti University of Medical Sciences , srmohebbi@rigld.ir 3- Shahid Beheshti University of Medical Sciences 4- دانشگاه علوم پزشکی شهید بهشتی
Abstract: (14901 Views)
ABSTRACT
Background and Aim: Production of unusual cytokine levels in hepatitis C infection appears to be associated with progression of the disease, persistence of the virus in host, and establishment of chronic disease. Interleukin-12 as a heterodimeric immunoregulating cytokine is important in the generation of a Th1-based immune response. In this study we investigated the role of IL-12B 3′UTR polymorphism in susceptibility to chronic hepatitis C infection.
Material and Methods: A total of 126 chronic hepatitis C patients and 136 healthy blood donors were genotyped for IL12-p 40- 3′ UTR polymorphism. Genotyping was carried out by PCR-RFLP method. The results were confirmed by analyzing 10 % of the samples by direct sequencing.
Results: We found no significant differences in genotype and allele frequencies of the 3’UTR polymorphism between chronic hepatitis C patients and healthy controls.
Conclusion: There was no association between IL12B-3’UTR polymorphism and chronic hepatitis C infection.Our study can be useful in regard to the factors regulating IL-12 production, and its consequent impact on chronic hepatitis C infection susceptibility in Iranian population.
Key words: Chronic hepatitis C, Polymorphism, IL12-p40, 3′UTR
Conflict of Interest: Nill
Received: Aug 16, 2010 Accepted: Dec 28, 2010
Azimzadeh P, Mohebbi S R, Romani S, Naghoosi H, Vahedi M, Kazemian S, et al . Effect of interleukin-12 p40 subunit gene 3’-untranslated region polymorphism in chronic HCV infection. SJKU 2011; 16 (1) :10-19 URL: http://sjku.muk.ac.ir/article-1-463-en.html