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Effect of interleukin-12 p40 subunit gene 3’-untranslated region polymorphism in chronic HCV infection
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Pedram Azimzadeh1   , Seyed Reza Mohebbi2 , Sara Romani3 , Hamed Naghoosi3 , Mohsen Vahedi3 , Shabnam Kazemian3 , Faramarz Derakhshan3 , Mohammad Reza Zali4 |
1- Shahid Beheshti Universityof Medical Sciences
2- Shahid Beheshti University of Medical Sciences , srmohebbi@rigld.ir
3- Shahid Beheshti University of Medical Sciences
4- دانشگاه علوم پزشکی شهید بهشتی |
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Abstract: (14901 Views) |
| ABSTRACT
Background and Aim: Production of unusual cytokine levels in hepatitis C infection appears to be associated with progression of the disease, persistence of the virus in host, and establishment of chronic disease. Interleukin-12 as a heterodimeric immunoregulating cytokine is important in the generation of a Th1-based immune response. In this study we investigated the role of IL-12B 3′UTR polymorphism in susceptibility to chronic hepatitis C infection.
Material and Methods: A total of 126 chronic hepatitis C patients and 136 healthy blood donors were genotyped for IL12-p 40- 3′ UTR polymorphism. Genotyping was carried out by PCR-RFLP method. The results were confirmed by analyzing 10 % of the samples by direct sequencing.
Results: We found no significant differences in genotype and allele frequencies of the 3’UTR polymorphism between chronic hepatitis C patients and healthy controls.
Conclusion: There was no association between IL12B-3’UTR polymorphism and chronic hepatitis C infection.Our study can be useful in regard to the factors regulating IL-12 production, and its consequent impact on chronic hepatitis C infection susceptibility in Iranian population.
Key words: Chronic hepatitis C, Polymorphism, IL12-p40, 3′UTR
Conflict of Interest: Nill
Received: Aug 16, 2010 Accepted: Dec 28, 2010 |
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| Keywords: Chronic hepatitis C, Polymorphism, IL12-p40, 3′UTR |
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Full-Text [PDF 1493 kb]
(2118 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2011/05/10 | Published: 2011/05/15
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