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:: Volume 24, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2019) ::
SJKU 2019, 24(1): 24-44 Back to browse issues page
Genetically modified mice- Methods, applications and outlook
Mahdi Eskandarian Boroujeni Dr.1 , Rafieh Alizadeh Miran Dr.2 , Fatemeh Afkhami Dr.3 , Zeinab Tavakol Dr.4 , Mehdi Shamsara Dr. 5
1- Department of Stem Cells and Regenerative Medicine, Faculty of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
2- ENT and Head & Neck Research center and department, Hazrat Rasoul Hospital, The five senses Institute, Iran University of medical sciences, Tehran, Iran
3- Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
4- Department of Midwifery, Faculty of Nursing and Midwifery, Shahrekord University of Medical Sciences, Shahrekord, Iran.
5- National Research Center for Transgenic Mouse, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , shamsa_m_2000@yahoo.com
Abstract:   (544 Views)
Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated the new methods developed to overcome these problems. Finally, we discussed future prospects on the gene targeting.
Methods & Materials: This is a review article, which has been written after searching Pubmed, Scopus, Google Scholar, Springer, Elsevier and Magiran databases by using keywords of transgenic mice, functional genetics, genetargeting, and homologous recombination.
Results: This study dealt with genetic variations in a wide range, differential processing and inactivation of gene-specific isoforms, local and induced genetic changes, Cre/loxP system and some future perspectives.
Conclusion: Success rate in genetic modification of mouse genome has increased dramatically, and use of knockout mice has resulted in increased knowledge of human biology and diseases.
Keywords: transgenic mice, functional genetics, target genes, homologous recombination
Full-Text [PDF 470 kb]   (212 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/12/1 | Accepted: 2019/03/25 | Published: 2019/03/25
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Eskandarian Boroujeni M, Alizadeh Miran R, Afkhami F, Tavakol Z, Shamsara M. Genetically modified mice- Methods, applications and outlook. SJKU. 2019; 24 (1) :24-44
URL: http://sjku.muk.ac.ir/article-1-4570-en.html


Volume 24, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2019) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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