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Genetically modified mice- Methods, applications and outlook
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Mahdi Eskandarian Boroujeni1    , Rafieh Alizadeh Miran2 , Fatemeh Afkhami3 , Zeinab Tavakol4 , Mehdi Shamsara 5 |
1- Department of Stem Cells and Regenerative Medicine, Faculty of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
2- ENT and Head & Neck Research center and department, Hazrat Rasoul Hospital, The five senses Institute, Iran University of medical sciences, Tehran, Iran
3- Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
4- Department of Midwifery, Faculty of Nursing and Midwifery, Shahrekord University of Medical Sciences, Shahrekord, Iran.
5- National Research Center for Transgenic Mouse, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , shamsa_m_2000@yahoo.com |
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Abstract: (3955 Views) |
Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated the new methods developed to overcome these problems. Finally, we discussed future prospects on the gene targeting.
Methods & Materials: This is a review article, which has been written after searching Pubmed, Scopus, Google Scholar, Springer, Elsevier and Magiran databases by using keywords of transgenic mice, functional genetics, genetargeting, and homologous recombination.
Results: This study dealt with genetic variations in a wide range, differential processing and inactivation of gene-specific isoforms, local and induced genetic changes, Cre/loxP system and some future perspectives.
Conclusion: Success rate in genetic modification of mouse genome has increased dramatically, and use of knockout mice has resulted in increased knowledge of human biology and diseases. |
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| Keywords: transgenic mice, functional genetics, target genes, homologous recombination |
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Full-Text [PDF 470 kb]
(3909 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2018/12/1 | Accepted: 2019/03/25 | Published: 2019/03/25
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