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:: Volume 23, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2018) ::
SJKU 2018, 23(3): 26-35 Back to browse issues page
Effect of Sambucus ebulus extract on neural stem cell prolifration under oxidative stress condition
Sara Haratizadeh 1, Mohammad Ali Ebrahimzadeh Dr2, Maryam Nazm Bojnordi Dr3, Hatef Ghasemi Hamidabadi Dr 4, Alireza Abdanipour Dr5, Javad Akhtari Dr6
1- Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- Associated Professor Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
3- Assistant Professor Department of Anatomy & Cell Biology, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Associated Professor Department of Anatomy & Cell Biology, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , hatefdr@gmail.com
5- Assistant Professor Department of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
6- Assistant Professor Immunogenetics Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abstract:   (210 Views)
Background and Aim: Recently, several studies have indicated that the central nervous system has the capacity for endogenous repair. But, the proliferation capacity of endogenous neural stem cells (NSCs) isn’t sufficient for the treatment of neurodegenerative diseases. So, it sounds that stimulation of endogenous NSC proliferation is essential for neuroregeneration. The aim of this study was to examine the effects of Sambucus ebulus extract on the proliferation of neonatal rat hippocampus-derived neural stem cells (NSCs) under oxidative stress condition induced by H2O2. 
Material and Methods: The NSCs were isolated from neonatal rat hippocampus. To confirm neural characteristics of neural stem cells, the expression of neural-specific marker, Nestin was investigated by immunocytochemistry technique. 5×104 cells were cultured in every well of a 96 well plate and H2O2 was added to induce oxidative stress condition. Then NSCs were exposed to 50 µg Sambucus ebulus extract for 24 hours, at various concentrations (25, 50, 100, 200, 400 and 500 μg/ml). The cell proliferation rate was assessed by MTT colorimetry assay before and after treatment with the extract.
Results: Immunofluorescent studies showed that neural stem cells expressed specific neural marker; Nestin. The proliferation rate of NSCs increased in the treated groups in comparison to that in the control group. The highest rate of survival was observed when Sambucus ebulus was used at the concentration of 500 μg/ml. (P<0.05).
Conclusion: The results showed that the methanolic extract of Sambucus ebulus can promote proliferation and survival of NCSs in vitro and also after exposure to oxidative stress condition, suggesting its potential beneficial effect on neuroregeneration.
Key Words: Neural stem cells, Sambucus ebulus, Proliferation, Survival.
 
Received: Jan 13, 2018     Accepted: Apr 11, 2018 
Keywords: Neural stem cells, Sambucus ebulus, Proliferation, Survival.
Full-Text [PDF 294 kb]   (93 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/07/31 | Accepted: 2018/07/31 | Published: 2018/07/31
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Volume 23, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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