[Home ] [Archive]   [ فارسی ]  
:: Volume 23, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2018) ::
SJKU 2018, 23(3): 1-9 Back to browse issues page
Investigation of the effect of trimethylamine-N-oxide on the proinflammatory cytokine genes expression in U937-derived macrophages
Mohammad Abdi Dr1, Zakarya Vahabzadeh Dr2, Amin Ghanivash 3, Leila Farhadi 4, Mohammad Saeed Hakhamaneshi Dr5, Pedram Andalibi Dr6
1- Assistant Professor Clinical Biochemistry Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Assistant Professor Liver & Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3- Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
4- Molecular Medicine and Genetics Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
5- Associated Professor Clinical Biochemistry Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
6- Instructor Clinical Biochemistry Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract:   (287 Views)

Background and Aim: Atherosclerosis is known as a multifactorial inflammatory disease. Trimethylamine N-oxide (TMAO) as a risk factor, has a potential to trigger or enhance the immune inflammatory reactions in atherosclerosis. Yet, The exact mechanism by which TMAO induces inflammation during atherosclerosis is not well understood. The present study was designed to evaluate the expression of IL-1β, IL-6, and TNF-α mRNA in response to treatment of macrophages with different concentrations of TMAO.

Material and Methods: In this experimental in-vitro study, U937-derived macrophages were treated with different concentrations of TMAO (37.5, 75,150 and 300 µM) for 24 h. A group of cells were also treated with tunicamycin as positive control for stress. RT-qPCR was used to evaluate the expression of IL-1β, IL-6, and TNF-α mRNA levels. One-Way ANOVA and Post-hoc Dunnett test were used to compare the mean value of every group with that of control group.

Results: Although TMAO increased expression of IL-1β, IL-6, and TNF-α mRNA, only 300 µM of TMAO significantly increased expression of IL-1β mRNA compared to the control cells (P<0.001). Tunicamycin increased expression of IL-6 significantly.

Conclusion: The results of this study showed that among the above mentioned cytokines, IL-1β as a proinflammatory cytokine had a greater role in inflammatory reactions, induced by TMAO as a risk factor for atherosclerosis.

Keywords: Cytokine, Atherosclerosis, Trimethylamine-N-Oxide, Tunicamycin, Macrophage.

 

Received: Nov 21, 2017     Accepted: May 15, 2018

Keywords: Cytokine, Atherosclerosis, Trimethylamine-N-Oxide, Tunicamycin, Macrophage.
Full-Text [PDF 263 kb]   (97 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/07/31 | Accepted: 2018/07/31 | Published: 2018/07/31
Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print



Volume 23, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 3742