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The effect of acetone extract of Moringa peregrina on Trichomonas vaginalis, Candida albicans and macrophage cells in vitro.
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Mina Ebrahimi1   , Javid Sadraei 2, Shahla Roudbarmohammadi3 , Fatemeh Nikoomanesh3 |
1- Department of Parasitology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
2- Department of Parasitology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran , Sadraeij@modares.ac.ir
3- Department of Mycology, Faculty of Medical Science, Tarbiat Modares University ,Tehran, Iran. |
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Abstract: (13914 Views) |
Background and Aim: Vulvovaginal candidiasis and trichomoniasis account for at least 50 percent of all cases of vaginal infections. The main drugs used to treat these infections have numerous side effects and drug resistance to them is on the rise. The aim of this study was to investigate the anti-trichomonas and anti candidal effects of acetone extracts of Moringa peregrina in vitro.
Material and Methods: We used acetone extracts of Moringa peregrina at concentrations of 375, 750, …, 3000 and 4000 µg/ml for the treatment of trichomoniasis; and concentrations of 0/003,…, 1 and 2mg/ml for the treatment of candidiasis. We evaluated the effect of the extracts after 24 and 48 hours.
The final number of viable parasites were determined by trypan blue staining and neobar lamella; and IC50 (50% Inhibitory Concentration) value was calculated. We also calculated MIC (Minimum Inhibitory Concentration) of the extract for candida. The cytotoxic effect of the extract on the mice macrophage cells was investigated.
Result: Comparison between treatment and control groups revealed a significant decrease in the viability of parasites in the treatment group at all concentrations after both 24 and 48 hours (P <0.05). After 24 hours the IC50 and SI values were calculated as 682 and 4.1 for parasite respectively and MIC value was 2 mg/ml for Candida albicans.
Conclusion: Considering favorable effects of acetone extract of Moringa peregrina on inhibiting the growth of Trichomonas vaginalis and Candida albicans identification and isolation of active ingredients of the plant, may lead to use of this extract for the treatment of both infections in the future studies.
Keywords: Trichomonas vaginalis, Candida albicans, Acetone extract of Moringa peregrina, Cytotoxicity, IC50, MIC.
Received: Jun 19, 2017 Accepted: Jan 8, 2018 |
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| Keywords: Trichomonas vaginalis, Candida albicans, Acetone extract of Moringa peregrina, Cytotoxicity, IC50, MIC. |
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Full-Text [PDF 524 kb]
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Type of Study: Original Research |
Subject:
General
Received: 2018/03/17 | Accepted: 2018/03/17 | Published: 2018/03/17
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