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Prevalence of phzM and pvdA virulence genes in pseudomonas aeruginosa isolated from Infectious disease ward patients
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Elahe Javid Rad1   , Fatemeh Keshavarzi2 |
1- Department of Microbiology, Sanandaj branch, Islamic Azad University, Sanandaj, Iran.
2- Department of Genetics, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran |
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Abstract: (4693 Views) |
Background and Aim: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and septicemia in patients with burn and cystic fibrosis. It is found in water and wet soil. PhzM and PvdA are virulence genes involved in the production of two iron carriers, pyoverdine and pyocyanin. Considering the importance of virulence genes in the bacteria, determination of the frequency of these genes in clinical and environmental samples has shown an increasing trend. The aim of this study was to investigate the prevalence of virulence genes in pseudomonas aeruginosa isolated from infected patients.
Material and Method: Clinical samples were obtained from the patients referring to Kermanshah hospitals. After isolation and identification of Pseudomonas aeruginosa strains, DNA extraction was performed by Sina Kit gene and the presence of genes evaluated by PCR. Data were analyzed by using SPSS v20 software.
Results: Among 106 strains, 34 (32.07%) and 47 (44.33%) strains were positive for pvdA and phzM genes, respectively. The logistic regression analysis revealed significant correlations between the presence of pvda gene and samples of urine (p<0.001), blood (p= 0.002), wound (p=0.004) and lung secretion (p=0.013). Foremore, there was no relationship between the presence of phzM gene and the above mentioned samples.
Conclusion: We found moderate prevalence rates for phzM and pvdA virulence genes in pseudomonas aeruginosa isolated from Kermanshah hospitals.
Key words: Pseudomonas aeruginosa, virulence genes, phzM, pvdA.
Received: Mar 15, 2017 Accepted: Sep 24, 2017 |
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| Keywords: Pseudomonas aeruginosa, virulence genes, phzM, pvdA. |
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Full-Text [PDF 3782 kb]
(1308 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2018/01/24 | Accepted: 2018/01/24 | Published: 2018/01/24
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