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:: Volume 22, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2017) ::
SJKU 2017, 22(5): 84-92 Back to browse issues page
Evaluation of astaxanthin effects on the survival and proliferation of human adipose derived stem cells
Nazem Ghasemi Dr *
Assistant Professor Department of Anatomical Science and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , n_ghasemi@med.mui.ac.ir
Abstract:   (661 Views)
Background and Aim: In order to enhance in vivo stem cell viability and considering similar anti-inflammatory and anti-apoptotic effects of human adipose derived stem cells (hADSCs) and astaxanthin (AST) and their ability to cross the blood-brain barrier, we investigated the effects of (AST) on hADSCs proliferation and viability to provide a supplement for cell based therapy in MS patients.
Materials and methods:  After isolation of hADSCs and assessment of CD markers, they were cultured in DMEM-F12 medium in the presence of AST at various concentrations (1, 5, and 10 ng/ml) for 72 h. Finally, we assessed cell proliferation and cell viability by MTT and Tryphan Blue methods.
Results: The results revealed that a high percentage of hADSCs expressed CD90 and CD44 markers and a low percentage of them expressed hematopoietic cell markers. In addition, in the group cultured in the presence of  5 ng/ml AST the mean percentage of cell viability increased significantly compared to other groups (p = 0.04).  Tryphan Blue results also revealed significant effect of AST on stem cell proliferation and culture of these cells in the presence of 5 ng/ml of AST, led to significant increase in the mean percentage of cell count compared to the results of the control group (p = 0.03).
Conclusion: Astaxanthin can increase hADSCs proliferation and survival and this agent can be used in the cell-based therapies in MS patients.
Key words: Astaxanthin, Cell proliferation, Cell survival.
 
Received: Apr 18, 2017     Accepted: Jul 15, 2017
Keywords: Astaxanthin, Cell proliferation, Cell survival.
Full-Text [PDF 261 kb]   (156 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/11/21 | Accepted: 2017/11/21 | Published: 2017/11/21
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Volume 22, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2017) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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