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:: Volume 21, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016) ::
SJKU 2016, 21(3): 11-25 Back to browse issues page
Survival and migration of bone marrow derived neurosphere after transplantation into subretinal space in albino rat AMD model
Hamid Abotaleb Kadkhodaeian1, Dr Takhi Tiraihi 2, Dr Hamid Ahmadieh3, Dr Hossein Ziaiiardakani4, Dr Narsis Daftarian5, Dr Taher Taheri6
1- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Professor Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran., (Corresponding Author), Tel:+98-21-82883895 , takialtr@modares.ac.ir
3- Professor Department of Ophthalmology, Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Associate Professor Department of Ophthalmology, Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5- Assistant Professor Department of Ophthalmology, Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6- Shefa neuroscience Research center, Khatam Al-Anbia Hospital, Tehran, Iran.
Abstract:  

Background and Aim: Degenerative retinal diseases are among the common causes of blindness in the world. The purpose of this study was to investigate transplantation of neurosphere derived from bone marrow tissue into subretinal space in age related macular degeneration induced by injection of sodium iodate in animal model.

Materials and Methods: 40 mg/kg of sodium iodate was injected into retro-orbital sinus of albino rats. Then histological investigation by flat-mount and hematoxylin and eosin staining was performed after 30 days. Bone marrow stromal stem cells isolated from albino rats femur, were cultured in the differentiation medium and induced into floating neurosphere. Differentiated cells were labeled with nuclear anti-BrdU and were transplanted into subretinal space. Seven days after injection, sections were prepared, and survival, migration and also arrangement of transplanted cells were investigated by immunohistochemistry.

Results: Three days after sodium iodate injection, the pathological changes such as increased autofluorescence, hypertrophy and multinuclearity in retinal pigmented epithelium were observed. Histological investigation showed disorganization of outer segment of photoreceptors and also changes in the retinal pigmented epithelium. Immunohistochemsitry findings, seven days after injection, showed that transplanted cells survived in subretinal space and could migrate into both retinal pigmented epithelium and the retinal layer and finally integrated with host tissue.

Conclusion: Due to accessibility, mesenchymal stem cells are regarded as a good source for transplantation. Potential of differentiation to neural linage and also survival ability and migration of these cells after transplantation could be regarded as a new way for the treatment of retinal degenerative diseases.

Keyword: Neurosphere cells, Bone marrow stromal stem cells, Sodium iodate, Age related macular degeneration.

Received: Dec 4, 2015      Accepted: Apr 26, 2016

Keywords: Neurosphere cells, Bone marrow stromal stem cells, Sodium iodate, Age related macular degeneration.
Full-Text [PDF 1020 kb]      
Type of Study: Research | Subject: General
Received: 2016/07/28 | Accepted: 2016/07/28 | Published: 2016/07/28
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Volume 21, Number 3 (Scientific Journal of Kurdistan University of Medical Sciences 2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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