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Genotyping of Mycobacterium tuberculosis isolates from TB patients with spoligotyping
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Rashid Ramazanzadeh   1, Noor Amirmozafari , Parisa Farnia , Farideh Ghazi |
| 1- , atrop_t@yahoo.com |
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Abstract: (14534 Views) |
| ABSTRACT
Background and Aim: Spoligotyping was applied to investigate the prevalence of all genotype in M. tuberculosis isolates. The associated risk factors among patients with different nationalities residing in Iran were also determined.
Materials and Methods: In this analytic cross-sectional study a total of 439 patients that referred to the NRITLD, the referral tuberculosis center in Iran have been registered during March 21st, 2003 to March 21st 2004.The isolated Mycobacterium tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. T-test and chi-square were used for analysis of the data.
Results: Spoligotyping of M. tuberculosis strains resulted in 140 different patterns that divided into 9 clades. One hundred twenty two (87.1%) of these spoligotype isolates were unique and reported for the first time. The remaining 18 (12.8%) spoligotype patterns were previously reported from other geographical regions of the world. Haarlem family was most prevalent than other genotype. Interestingly, 6.3% of the strains belonged to the Beijing family. The MDR (multi drug resistance), double and triple resistance were seen in group Ι of evolutionary scenario. Antibiotic resistances were higher in those isolated from the Afghani patients (p<0.001). The other risk factors such as sex and age were also contributing factors to the diseases state.
Conclusion: The results showed that multi drug-resistance was more prevalent in bacteria isolated from Afghani TB patients residing in Iran. In addition, spread of M. tuberculosis strains belonging to the Beijing family among Iranian patients has to be considered seriously. It is also important to undertake studies to identify which factors are the most significant to consider in tuberculosis control program.
Key words: Tuberculosis, Resistance, Antibiotics, Spoligotyping, Beijing |
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| Keywords: Tuberculosis, Resistance, Antibiotics, Spoligotyping, Beijing |
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Full-Text [PDF 269 kb]
(2141 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2009/10/27 | Published: 2006/06/15
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