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Effect of chloroformic extract of Cichorium intybus on liver function tests and serum level of TNF-α in obstructive cholestasis in rat
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Mohammad Raman Moloudi1   , Kambiz Hassanzadeh1 , Shamileh Rouhani2 , Farid Zandi1 , Abbas Ahmadi1 , Pooneh Khalwatian1 , Amin Rostami2 , Farshad Sheikh Esmaeili1 , Esmael Izadpanah 3 |
1- Kurdistan University of Medical Sciences
2- Kurdistan University
3- Kurdistan University of Medical Sciences , eizadpanah2000@yahoo.com |
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Abstract: (8252 Views) |
| Background and Aim: Liver plays important roles in the production of bile, detoxification, and elimination of foreign material and synthesis of plasma proteins. Obstructive cholestasis is one of the liver disorders that can result in increased concentration of oxidants and inflammatory agents in the liver. In traditional medicine, Cichorium intybus has been used as a liver protectant, anti inflamatory and detoxifying agent. The aim of this study was to evaluate the effect of chloroformic extract of Cichorium intybus on liver functional tests and serum level of TNF-α in cholestatic rat model.
Materials and Methods: In this experimental study, male Wistar rats were randomly divided into 5 groups (n= 6) including sham operated, control (Bile Duct Ligation (BDL) + vehicle), and 3 groups with BDL + extract treatments (100, 200, 400 mg/kg/day ip). These groups were treated for seven days and on the eighth day, prothrombin time (PT), serum albumin, alanine amino transferase (ALT), aspartate amino transferase (AST), total bilirubin, direct bilirubin, alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), were measured by calorimetric and TNF-α was measured by ELISA methods. The results were analyzed by one way ANOVA.
Results: The results of the present study showed that the Cichorium extract (100 mg/kg/day) decreased the serum level of direct bilirubin, AST, ALT, and TNF-α significantly compared to the control group (p <0.05). Furthermore, at the higher dose (200 mg/kg/day) PT, ALP, LDH and AST decreased significantly in comparison to the control group (p <0.05), while the serum albumin level increased significantly in the treated animals (p <0.05).
Conclusion: In conclusion, we found that, low doses of chloroformic extract of Cichorium intybus protected the liver against obstructive cholestasis induced -injury.
Key words: Cichorium intybus, Obstructive cholestasis, Liver function tests.
Received: Apr 29, 2014 Accepted: Jun 28, 2014 |
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| Keywords: Cichorium intybus, Obstructive cholestasis, Liver function tests. |
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Full-Text [PDF 348 kb]
(5470 Downloads)
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Type of Study: Original Research |
Subject:
General
Received: 2014/12/17 | Accepted: 2014/12/17 | Published: 2014/12/17
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