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Showing 6 results for keshavarzi
Latifeh Mohammadpour, Dr Mohammad Sadegh Fallah, Dr Fatemeh Keshavarzi, Dr Siroos Zeinali, Dr Bayazeed Ghaderi, Dr Azad Fattahy Rad, Dr Reza Akramipour, Sara Azadmehr, Volume 20, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Alpha Thalassemia is an autosomal recessive genetic disease by reduced or absent synthesis of alpha globin polypeptide chains. One of the severe forms of disease with 3 alpha globin genes inactivation is H disease. In this study genotype of H disease was assessed in some patients with blood abnormalities referred to hospitals of Kermanshah and Kurdistan provinces. Materials and method: In this descriptive research, 110 patients with microcytic and hypochromic anemia were recruited. Based on CBC and hemoglobin electrophoresis tests in patients before blood transfusion or their parents, alpha thalassemia was diagnosed. DNA was extracted by salting out method. The common deletions, alpha globin point mutations and direct sequencing were investigated using multiplex PCR and Sanger methods respectively. Results: In twelve alpha thalassemia patients, it has been shown the deletion mutations of --Med in 5 patients (20.8%), -α3.7 in 4 patients (6.6%) and -α20.5 in 3 patients (12.5%) and also point mutations polyA1 in 3 patients (12.5%),-αcd59 in 2 patients (8.3%) and -αIVSI (-5nt) in 2 patients (8.3%). In 4 cases deletion (-α/--) and in 3 cases (40%) non-deletion H disease (--/ααT) were diagnosed. Two cases showed point mutation of ααT/ααT. Two patients were blood-transfusion dependent. The first one who received regular blood monthly, showed --Med/αcd59 genotype. The second one with -α20.5/-α3.7 genotype received blood occasionally. In 7 patient’s enlargement of spleen was observed and in 3 patients splenectomy was performed earlier. Conclusion: The results showed the genetic diversity in alpha-globin and the importance of point mutations in the H disease. However, further study should be done to determine the accurate genotype and phenotype relationship and to diagnose of H disease in prenatal cases. Keywords: Alpha Thalassemia, H disease, Kurdistan, Kermanshah. Received: Jul 16, 2014 Accepted: Feb 7, 2015
Arezoo Darabi, Dr Fatemeh Keshavarzi, Bahareh Sedaghatikhayat, Pezhman Salehifar, Mahboobeh Masoudifar, Dr Azad Fattahy Rad, Dr Siroos Zeinali, Dr Mohammad Sadegh Fallah, Volume 20, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2015)
Abstract
Background and Aim: Beta-thalassemia is a common autosomal recessive disorder of hemoglobin synthesis. About 200 mutations of beta globin genes have been detected .This study was performed to determine beta globin gene mutations in blood transfusion dependent patients in Kurdistan Province hospitals from June 2012 to April 2014 .
Material and Methods: This descriptive study included sixthy eight blood transfusion dependent patients with beta- thalassemia major who had referred to Kurdistan Province hospital.Patients and their parents completed our questionnaires. Based on CBC and hemoglobin electrophoresis results diagnosis of beta thalassemia was made. 5 ml blood was obtained from every participant and DNA was extracted by using standard salting out method. Then we investigated beta globin gene mutations by ARMS-PCR method and DNA sequencing.
Results: The most prevalent mutations were IVS-II-1 in 30 allels (22.5%), Fr8-9(+G) in 22 allels (15.94%), IVS-I-1 in 13 allels (9.42%) and C36/37 in 11 allels (7.97%). Among 138 allels, types of mutations in 42 allels of assessed 138 allels remained unknown.
Conclusion: The results of this study were compatible with those of another study performed in Sanandaj in 2003, but in our study, mutation of C36/37 was more prevalent.
Keywords: Beta thalassemia, ARMS-PCR, Kurdistan
Received: Sep 28, 2014 Accepted: Jul 6, 2015
Azin Ashja Ardalan, Dr Fatemeh Keshavarzi, Dr Azad Fattahy Rad , Dr Fahimeh Baghbani-Arani , Volume 21, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2016)
Abstract
Background and Aim: Increased resistance to the macrolides is probably due to excessive and inappropriate use of this type of antibiotics. The purpose of this study was to investigate macrolide resistance in group A Streptococci strains isolated from clinical samples carrying mef gene and comparison of the phenotype and genotype of the mef gene carriers and prevalence of bacteria in the study population.
Material and methods: Forty Beta-hemolytic streptococci pyogenes group A isolates obtained from 825 various clinical specimens by using disk agar diffusion (DAD) were studied for the presence of mefA and mefE genes by polymerase chain reaction (PCR). Statistical analysis was performed by means of SPSS and Microsoft Office Excel softwares.
Results: Prevalence of group A streptococcus was 4.84 % and also we found a macrolide resistance of 40% among isolates in the study population. By using D-test, the prevalence rates of phenotypes of GAS resistant to erythromycin among 8 isolates were 50% (4cases) for M-phenotyp,37.5% (3 cases) for cMLS- phenotype and 12.5% (1 case) for iMLSB -phenotype 1. Among 16 isolates resistant to macrolides, 8 (50%) had mefA, one (6.25%) had mefE and 7 isolates (43.75%), didn,t have mefA / E genes.
Conclusion: In the present study M- phenotype had the highest frequency among GAS isolates resistant to erythromycin. Also, PCR can be used as a confirmatory routine test for interpretation of the results of anti-biogram and D-tests.
Keywords: Beta-hemolytic streptococci group A, Macrolides, MLSB, D-TEST, Gene mef.
Received: Jul 27, 2015 Accepted: Jun 21, 2016
Monireh Alipour, Dr Leila Ghahremani, Dr Sedigheh Amooee, Dr Sareh Keshavarzi, Volume 22, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2017)
Abstract
Background and Aim: Psychosocial variables in pregnant women, including prenatal stress, anxiety and depression are associated with maternal, infant and child health. Relaxation technique is an intervention that increases comfort and might be effective in reducing psychological tension in pregnant women.
The aim of the present study was to evaluate the effects of relaxation techniques training, based on self-efficacy theory, on depression, anxiety and stress in pregnant women
Material and Methods: In this interventional study, 78 pregnant women were randomly divided into experimental and control groups. The intervention group learned relaxation techniques in four sessions, after doing exercises at home for 4 weeks. However, the control group performed no interventions. The data were collected using demographic information, Depression Anxiety Stress Scale (DASS 21) and self-efficacy scale at the baseline and one month after the intervention. Using SPSS version 19 data were analyzed by descriptive and inferential statistics.
Results: The results of this study showed significant decrease in depression (p<0.001), anxiety (p<0.001) and stress (p<0.001) in the pregnant women in the intervention group, but no significant difference was observed in the control group. Moreover, a significant difference was found in the mean scores of self-efficacy in the intervention group before and after intervention (p< 0.001).
Conclusions: The results of this study supported the claim that training relaxation techniques could reduce stress, anxiety and depression in pregnant women through its effect on coping mechanism, leading to promotion of well-being. We recommend inclusion of perinatal meditation into perinatal care and counseling programs.
Keywords: Relaxation, Stress, Anxiety, Depression, Pregnancy.
Received: Jul 11, 2016 Accepted: Apr 16, 2017
Elahe Javid Rad, Dr Fatemeh Keshavarzi, Volume 22, Issue 6 (Scientific Journal of Kurdistan University of Medical Sciences 2018)
Abstract
Background and Aim: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and septicemia in patients with burn and cystic fibrosis. It is found in water and wet soil. PhzM and PvdA are virulence genes involved in the production of two iron carriers, pyoverdine and pyocyanin. Considering the importance of virulence genes in the bacteria, determination of the frequency of these genes in clinical and environmental samples has shown an increasing trend. The aim of this study was to investigate the prevalence of virulence genes in pseudomonas aeruginosa isolated from infected patients.
Material and Method: Clinical samples were obtained from the patients referring to Kermanshah hospitals. After isolation and identification of Pseudomonas aeruginosa strains, DNA extraction was performed by Sina Kit gene and the presence of genes evaluated by PCR. Data were analyzed by using SPSS v20 software.
Results: Among 106 strains, 34 (32.07%) and 47 (44.33%) strains were positive for pvdA and phzM genes, respectively. The logistic regression analysis revealed significant correlations between the presence of pvda gene and samples of urine (p<0.001), blood (p= 0.002), wound (p=0.004) and lung secretion (p=0.013). Foremore, there was no relationship between the presence of phzM gene and the above mentioned samples.
Conclusion: We found moderate prevalence rates for phzM and pvdA virulence genes in pseudomonas aeruginosa isolated from Kermanshah hospitals.
Key words: Pseudomonas aeruginosa, virulence genes, phzM, pvdA.
Received: Mar 15, 2017 Accepted: Sep 24, 2017
Zakieh Keshavarzi, Mosa-Al- Reza Hadjzadeh, Masoud Nazari, Roghaye Arezumand, Volume 26, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2021)
Abstract
Aim and Background: Anti-inflammatory compounds are effective in improving the symptoms of inflammatory bowel diseases such as ulcerative colitis. The aim of this study was to evaluate the effect of aloe vera extract against the inflammatory factors affecting acetic acid-induced ulcerative colitis.
Materials and Methods: 32 male Wistar rats are divided into 4 groups of 8 including: control group, untreated colitis group, colitis group treated with aloe vera (200 mg / kg/ day) and sulfasalazine group as a standard treatment (500 mg /kg/ day) from two days before induction of colitis to 5 days after induction of colitis. Experimental colitis was induced by intrarectal injection of 2 ml of 3% acetic acid. Rats were sacrificed on the eighth day of induction of colitis and some factors involved in inflammation, such as tumor necrosis factor-alpha, interleukin-10, cyclooxygenase 2 (COX II) and PGE2 levels in colon tissue were measured.
Results: Inflammation induced in colon by acetic acid injection. Sulfasalazine and aloe vera reduced the inflammatory factor (tumor necrosis factor alpha) and increased anti-inflammatory factor (interleukin-10) in colon tissue. But they had no effect on the COX II and PGE2 levels in colon tissue.
Conclusion: The results suggested that aloe vera could possibly improve colitis by suppressing inflammatory promoting factors and increasing anti-inflammatory factors.
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