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Journal Citation Index

 

Citation Indices from GS

AllSince 2020
Citations102885691
h-index3925
i10-index271142

 

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Central Library of Kurdistan University of Medical Sciences
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Vice-Chancellery for Research and Technology
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Showing 4 results for Hosseinpour Feizi

Sh Khorshidi, M Haghi, Dr M.a Hosseinpour Feizi, Dr M Aminbakhsh, Dr A Hosseinpour Feizi, N Pouladi,
Volume 13, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2008)
Abstract

  ABSTRACT

  Background and Aim: β -thalassemia is the most common autosomal recessive disorder. More than 200 different mutations in the β-globin gene have been detected which can lead to decreased or absent β -globin chain synthesis . Since the Iranian population is a mixture of different ethnic groups, it is necessary to determine the frequency and distribution of these mutations in the different ethnic groups of our country. Therefore, in this study we determined the spectrum and the frequency of β-thalassemia mutations in the patients with β-thalassemia major in the Kurd population of Kurdistan and West Azerbaijan provinces of Iran.

  Material and Methods: To detect mutations, extracted DNA of 110 chromosomes from 55 unrelated patients, were studied by PCR-ARMS (Polymerase Chain Reaction-Amplification Refractory Mutation System), SSCP (Single Strand Conformation Polymorphism) and direct sequencing methods.

  Results: The results of this study showed that IVS-II-1 ¨ (G→A) was the most common mutation with a frequency of 31% FSC * 8/9(+G) with a frequency of 19% was the second most prevalent mutation among all chromosomes. Other mutations were IVS-I-1(G→A), FSC8 (-AA), IVS-I-110(G→A), FSC36/37(-T), IVS-I-5(G→C), IVS-I-128(T→G), FSC44 (-C), FSC 5(-CT) and +22UTR · (G→A). These mutations comprised 79 % of β -thalassemia mutations in this region and 21% of the mutations still remains to be explored.

  Conclusion: The results of this study showed that, there are similarities and differences between this region and other parts of Iran and also neighboring countries. Therefore, determination of β-thalassemia mutations in this region seems to be necessary and beneficial for designing prenatal diagnosis programs.



  ¨ . Intervening Sequence-II-nucleotide 1

  * . Frame Shift Codon

  · . Untranslated Region


Kayhane Kyani, Dr Mohammad Ali Hosseinpour Feizi , Esmail Babaei, Dr Vahid Montazeri, Dr Monireh Halimi,
Volume 14, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2009)
Abstract

ABSTRACT Background and Aim: Thyroid carcinoma is the most common endocrine malignancy. Considering highly heterogenous nature of tumoral and non-tumoral thyroid nodules from pathological point of view and also in regard to absence of appropriate molecular markers, extensive efforts have been made to find a molecular tumor marker for specific diagnosis of thyroid tumors. Recent attention has been paid to Survivin, a new member of the Inhibitor of Apoptosis Protein Family (IAP), as a new molecular marker in cancer. Studies have been demonstrated that Survivin and its splice variants have different expressions in cancerous tissues compared to normal tissues. In this study the expression of Survivin-2α splice, one of the newest Survivin variants, was evaluated in thyroid cancer as a molecular marker. Materials and Methods: Tissue samples were collected from 77 thyroid specimens including 49 tumoral, 14 nontumoral and 14 tumor margin samples. The expression of Survivin-2α was studied by Hemi-Nested RT-PCR method. Results: Expression of Survivin-2α splice was the highest in surgical margin samples compared to non-tumoral and tumoral samples. The lowest expression was that of tumoral samples. Conclusion: Our data demonstrated the expression of Survivin-2α in thyroid tumors. Although the expression of surviving-2α splice variant in tumoral cells was lower than that of tumor margins, it did not show a significant difference. Therefore it seems likely that it does not have a special role in the progression of tumor and development of abnormal nature of the cells. According to the results of this study, it can be concluded that the different expressions of 2α in these groups can not be an appropriate criterion for distinguishing tumors from non-tumoral lesions of thyroid gland. Key words: Thyroid cancer, Survivin-2α, Splice Variants, Hemi-nested RT-PCR Conflict of Interest: Nill Received: June 6, 2009 Accepted: October 27, 2009
Naser Pooladi, Dr Mohammad Ali Hosseinpour Feizi , Mehdi Haghi, Parvin Azarfam, Dr Abbasali Hosseinpour Feizi,
Volume 15, Issue 3 (Scientific Journal of Kurdistan University of Medical Sciences 2010)
Abstract

ABSTRACT Background and Aim: β-thalassemia (β-thal) is one of the most prevalent hereditary diseases in Iran. There are more than two million carriers of β-thal in Iran. Detection of the beta globin gene mutations is necessary for a definitive diagnostic and management plan such as prenatal diagnosis of β-thalassemia. In our country, the PCR-Amplification Refractory Mutation System (PCR-ARMS) has been frequently used for detection of beta globin gene mutations. Material and Methods: Here, we used the PCR-single strand conformation polymorphism (PCR-SSCP) assay for detection of mutations of beta globin gene. In the patients with confirmed mutations, we amplified 281base pairs containing exon of one of a beta globin gene by PCR. Based on SSCP technique 2.5 µl of the reaction products appeared in polyacryamide gel electrophoresis and the bands were visualized by silver staining. Seven mutations and one polymorphism were evaluated by PCR-SSCP assay. Results: The results of this study demonstrated that the patterns of mobility of single strands were different from each other and those of control sample. Conclusion: Our study showed the PCR-SSCP technique can meet the need for direct genomic sequencing of DNA and could be applied in the developing countries where financial resources are limited but genetic hemoglobin disorders are highly prevalent. Key words: Thalassemia, SSCP, β- globin gene. Conflict of Interest: Nill Received: June 12, 2010 Accepted: Nov 11, 2010
Keihaneh Kiani, Dr Mohammad Ali Hosseinpour Feizi, Dr Esmail Babaie, Dr Vahid Montazeri, Dr Monireh Halimi,
Volume 17, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012)
Abstract

ABSTRACT Background and Aim: Survivin is a new member of inhibitor of apoptosis protein family (IAP) that plays an important role in the regulation of cell cycle and inhibition of apoptosis. Distinct expression of this gene in tumoral cells versus normal cells introduces it as the fourth major transcriptome in cancers. Thyroid carcinoma is the most common endocrine malignancy. Considering the highly heterogeneous nature of tumoral and non-tumoral thyroid nodules from the pathological viewpoint and also in regard to the absence of appropriate molecular markers, extensive efforts have been made to find a specific molecular tumor marker for diagnosis of thyroid tumors. Studies have been demonstrated that the expression pattern of survivin and its splice variants was different in cancerous tissues compared to normal tissues. In this study we evaluated expression of survivin-3b and survivin-3α, the novel survivin splice variants, as diagnostic markers for thyroid cancer. Materials and Method: This was a descriptive study. 77 thyroid specimens including 49 tumoral, 14 non-tumoral and 14 tumor margin samples were collected and expression of survivin-3b ands-3α was investigated by hemi-nested RT-PCR method. Results: Tumoral samples showed the highest expression of survivin-3b and survivin-3α and the lowest expression was detected in the specimens of tumor margins. Conclusion: In this study we demonstrated the expression of survivin-3b and survivin-3α in thyroid tumors for the first time. In conclusion significant expression of survivin-3b and survivin-3α splice variants in tumoral cells shows their roles in thyroid cancer progression and their efficiency as molecular markers for detection and classification of tumoral and non-tumoral thyroid nodules. Key words: Thyroid cancer, Survivin-3b، Survivin-3α, Splice variant, Hemi-nested RT-PCR Received: Aug 15, 2011 Accepted: Aug 30, 2012

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مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
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