|
|
|
 |
Search published articles |
 |
|
Showing 1 results for Haji Esmaili Qomi
Ali Haji Esmaili Qomi, Mahtab Noorifard, Ramin Hamidi Farahani, Mehdi Shakori Khomartash, Abbas Morovvati, Ashkan Dirbazian, Pegah Shakib,
Volume 28, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2023)
Abstract
Background and Aim: Covid-19 disease is a global health concern that requires rapid quantitative diagnostic testing. The standard technique for diagnosing SARS-CoV-2 is now REAL-TIME PCR. The aim of this study was to design a sensitive Reverse Transcription Loop-Mediated Isothermal Amplification method for the early identification of SARS-CoV-2.
Material and Methods: In this laboratory study, specimens were prepared from the patients by laryngeal, pharyngeal, and nasal swabs and after RNA extraction and cDNA synthesis. SARS-CoV-2 virus was evaluated using the RT-LAMP method. Sensitivity of the RT-LAMP method was evaluated by primers targeting N and E genes.
Results: In this study, 19 SARS-CoV-2 RNA samples were examined. According to the results of this study turbidity or discoloration of fluorescent dye showed that agarose electrophoresis gel is a more sensitive test. Detection limits for the number of E gene copy were observed at the dilutions of 10-9 and 10-8 for gel electrophoresis and fluorescent light, respectively. This means detection of 1 copy and 14 copies for the two above-mentioned methods, respectively. However, in the case of the N gene the detection limits, were at the dilutions of 10-10 on the gel and 10-8 by fluorescent light, which indicated detection of 1 copy and 17 copies, respectively. The calculations were performed using the Chiang formula.
Conclusion: The results of this study showed that the LAMP was a simple, fast, sensitive, and specific method for the diagnosis of SARS-CoV-2 that may improve the diagnostic potential in clinical laboratories.
|
|
