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Showing 3 results for Barati
Dr Bohlool Habibi-Asl, Dr Amir Hooshang Barati, Dr Nazila Darvishi, Dr Esmael Izadpanah, Mehdi Mafakheri, Dr Kambiz Hasanzadeh,
Volume 16, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2012)
Abstract
ABSTRACT
Background and Aim: Long – term use of opiates induces tolerance to the analgesic effect. Despite significant investigations, the precise cellular mechanisms underlying opioid tolerance is not clear. Many studies have revealed the key role of nitric oxide in the morphin-induced tolerance. The aim of this study was to evaluate the effects of nicorandil (a nitric oxide donor and ATP sensitive potassium channel opener) and glibenclamide (an ATP sensitive potassium channel blocker) on morphine-induced tolerance.
Materials and Methods: In this study male mice weighting (20-30g) were randomly placed into groups of 8, and received different therapeutic regimens for 5 days. Different groups received either morphine (50mg/kg, i.p) + normal saline (10ml/kg, i.p), or morphine (50mg/kg, i.p) + nicorandil (2.5, 5, 10mg/kg, i.p) or morphine (50mg/kg, i.p) + glibanclamide (5, 10, 15mg/kg, i.p) every day. Nociception was assessed using a hotplate apparatus on the 6th day. The nociceptive effect was recorded when the animal licked its hind paw or jumped due to the heat effect.
Results:Our results showed that tolerance to the analgesic effect of morphine significantly increased in the group which received morphine + nicorandil (5, 10mg/kg, i.p), (p<0.05), while in morphine + glibenclamide group, tolerance significantly reduced (p<0.05).
Conclusion: The results of this study indicated that intraperitoneal injection of nicorandil increased tolerance to the analgesic effects of morphine while glibenclamide decreased tolerance. The above effect seems to be related to the role of nitric oxide (NO) and ATPsensitive potassium channel in this phenomenon.
Key word: Morphine, Nicorandil, Glibenclamide, Tolerance.
Received: Jan 9, 2011 Accepted: Nov 5, 2011
Conflict of interest: Nill
Sara Barati, Dr Hossein Najafzadehvarzi, Dr Dariush Gharibi,
Volume 22, Issue 4 (Scientific Journal of Kurdistan University of Medical Sciences 2017)
Abstract
Background and Aim: Considering bacterial resistance to common antibiotics and the need for new drugs, use of medicinal products manufactured by nano-technology, can be effective in the prevention and treatment of bacterial infections. In this study, we evaluated the sensitivity of Staphylococcus aureus and Salmonella to nanoparticles of magnesium oxide and silicon oxide in vitro.
Methods: Staphylococcus intermedius and Methicillin-resistant Staphylococcus aureus and Salmonella typhimurium, Salmonella abourtus were cultured in Mueller Hinton Broth medium. Then different concentrations of the nanoparticles of magnesium oxide and silicon oxide were added to the culture medium. After 24 hours of incubation we measured optical density (OD) by means of ELISA reader. Multi-well plate was used as controls. Using SPSS software data were analyzed by ANOVA and LSD post hock test.
Results: Silicon nanoparticles prevented growth of Staphylococcus intermedious, Staphylococcus aureus, Salmonella typhimurium and Salmonella abortus (p<0.0001) in a dose dependent way, but showed no antibacterial effect on Salmonella typhi. Also nanoparticles of magnesium oxide showed antimicrobial effect on the above-mentioned bacteria in a dose-dependent manner (p<0.0001).
Conclusion: Silicon oxide and magnesium oxide nanoparticles can be used and evaluated as antibacterial drugs in experimental or clinical infections.
Keywords: Nanoparticles of silicon, Magnesium oxide nanoparticle, Antibacterial, Methicillin resistant bacteria.
Received: May 23, 2016 Accepted: Dec 21, 2016
Adel Ghaderi, Mohammad Bagher Kkhadem Eerfan, Mohammad Barati, Shahla Ghaderi,
Volume 23, Issue 5 (Scientific Journal of Kurdistan University of Medical Sciences 2018)
Abstract
Background and Aim: Leishmaniasis is a zoonotic disease which presents with a wide range of clinical features, including cutaneous and visceral forms in Iran. Leishmania (L) major is one of the agents responsible for cutaneous leishmaniasis transmitted by the bite of sand fly. In this study we assessed the antileishmania effect of pistacia Atlantica (alpha-pinene) in culture and also its therapeutic effects on Balb/c mice infected with L. Major.
Material and Method: Certain amount of promastigots was challenged with increasing concentrations of pistacia atlantica extract. MTT test was used to assess promastigote survival after 24, 48 and 72 hours. For in vivo assessment, in the stationary phase, 0.1 ml solution containing 2×106 promastigotes were injected subcutaneously into the base of the tails of the mice. Four weeks after injection, cutaneous lesions appeared and different doses of the extract were applied daily in the form of an ointment for 3 weeks. Diameters of the lesions were measured at the end of each week and therapeutic effect of the extract on the lesions was assessed.
Results: The results of MTT test revealed remarkable effect of the treatment on the growth of promastigots. IC50 values for glucantime and alpha pinene were found to be 10 µg/ml and 1.46 µg/ml respectively. 30 % ointment of the extract decreased the lesion diameter significantly while 15% ointment and treatment for control group were ineffective.
Conclusion: The results of the present study showed antileishmanial effect of alpha-pinene on Leishmania major promastigots, in vitro. Moreover, topical ointment of the extract can reduce size of the lesions caused by the parasite, in vivo.
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