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Effect of Acute Administration Caffeine and Adenosine Alone or in Combination with Celecoxib on Pentylenetetrazole (PTZ)- induced Seizures in Mice
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Marzieh Bazmandegan1    , Azhdar Heydari 2 |
1- Kashan University of Medical Sciences & Kashan University of Medical Sciences
2- Kashan University of Medical Sciences & Kashan University of Medical Sciences , heydariazh@mail.com |
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Abstract: (374 Views) |
Background and Aim: The aim of this study was to investigate the effect of acute administration of caffeine and adenosine alone or in combination with celecoxib (a cyclooxygenase-2 inhibitor) on the seizure threshold induced by pentylenetetrazole (PTZ).
Materials and Methods: Male NMRI mice (30-25 g, 10 per group) were divided into groups of 10, control and experimental groups receiving adenosine (25, 50, and 100 mg/kg), caffeine (10, 40, 80, and 100 mg/kg), celecoxib (2.5, 5, and 10 mg/kg) and combination of celecoxib with adenosine or caffeine. The threshold doses for clonic and tonic seizures were measured by intravenous infusion of PTZ.
Results: Adenosine at doses of 50 and 100 mg/kg showed anticonvulsant effects. Caffeine at the doses of 10, 40, 80, and 100 mg/kg significantly reduced the tonic seizure threshold while only at the doses of 10 and 40 mg/kg reduced the clonic seizure threshold. Celecoxib had anticonvulsant effects only at the dose of 10 mg/kg. The combination of celecoxib with adenosine enhanced the anticonvulsant effect of adenosine. In addition, the combination of celecoxib with caffeine reduced the proconvulsant effects of caffeine to some extent.
Conclusion: The combination of celecoxib with caffeine abolished some of the convulsant effects of caffeine and potentiated the anticonvulsant effect of adenosine, suggesting that part of the convulsant effect of caffeine and anticonvulsant effect of adenosine was through modulation of the cyclooxygenase-2 pathway.
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| Keywords: Caffeine, Cyclooxygenase, Celecoxib, Adenosine, Seizure |
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Full-Text [PDF 965 kb]
(68 Downloads)
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Type of Study: Original Research |
Subject:
Physiology and neurosciences
Received: 2023/06/10 | Accepted: 2023/10/25 | Published: 2024/09/18
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